Dna sequencing by synthesis using modified nucleotides and nanopore detection

ABSTRACT

This disclosure is related to a method of sequencing a single-stranded DNA using deoxynucleotide polyphosphate analogues and translocation of tags from incorporated deoxynucleotide polyphosphate analogues through a nanopore.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. application Ser. No. 16/653,278, filed Oct. 15, 2019, now allowed, which is a divisional of U.S. application Ser. No. 13/994,431, filed Oct. 22, 2014, now U.S. Pat. No. 10,443,096, issued Oct. 15, 2019, a § 371 national stage of PCT International Application No. PCT/US2011/065640, filed Dec. 16, 2011, claiming priority of U.S. Provisional Application Nos. 61/424,480, filed Dec. 17, 2010, and 61/557,558, filed Nov. 9, 2011, the contents of each of which are hereby incorporated by reference in their entirety.

Throughout this application, certain patents and publications are referenced, the latter by authors and publication year. Full citations for these publications may be found immediately preceding the claims. The disclosures of these patents and publications in their entireties are hereby incorporated by reference into this application in order to describe more fully the state of the art to which this invention relates.

BACKGROUND OF THE INVENTION

DNA sequencing is a fundamental technology for biology. Several analytical methods have been developed to detect DNA or RNA at single molecule level using chemical or physical microscopic technologies [Perkins et al. 1994, Rief et al. 1999, Smith et al. 1996, and Vercoutere et al. 2001].

In the past few years, ion-sensing technologies such as ion channel, which relies on the detection of hydrogen ion (H⁺) released when a nucleotide is incorporated into a strand of DNA by a polymerase [Rothberg et al. 2011], have been explored to detect individual DNA or RNA strands [Kasianowicz 2003 & 2004, Chandler et al. 2004, Deamer et al. 2002, Berzukov et al. 2001, and Henrickson et al. 2000].

It has been demonstrated that an α-hemolysin channel, an exotoxin secreted by a bacterium, can be used to detect nucleic acids at the single molecule level [Kasianowicz et al. 1996]. An α-hemolysin protein is a monomeric polypeptide which self-assembles in a lipid bilayer membrane to form a heptameric pore, with a 2.6 nm-diameter vestibule and 1.5 nm-diameter limiting aperture (the narrowest point of the pore) [Meller et al. 2000, Akeson et al. 1999, and Deamer et al. 2002]. The limiting aperture of the nanopore allows linear single-stranded but not double-stranded, nucleic acid molecules (diameter ˜2.0 nm) to pass through. In an aqueous ionic salt solution such as KCl, when an appropriate voltage is applied across the membrane, the pore formed by an α-hemolysin channel conducts a sufficiently strong and steady ionic current. The polyanionic nucleic acids are driven through the pore by the applied electric field, thus blocking or reducing the ionic current that would be otherwise unimpeded. This process of passage generates an electronic signature (FIG. 1 ) [Vercoutere et al. 2001 and Deamer et al. 2002]. A particular nucleic acid molecule, when entering and passing through the nanopore generates a characteristic signature that distinguishes it from other nucleic acid molecules. The duration of the blockade is proportional to the length of nucleic acid, and the signal strength is related to the steric and electronic properties of the nucleotides, namely the identity of the four bases (A, C, G and T). Thus a specific event diagram, which is a plot of translocation time versus blockade current, is obtained and used to distinguish the length and the composition of polynucleotides by single-channel recording techniques based on characteristic parameters such as translocation current, translocation duration, and their corresponding dispersion in the diagram [Meller et al. 2000].

It has also been shown that a protein nanopore with a covalently attached adaptor can accurately identify unlabeled nucleoside 5′-monophosphates (dAMP, dGMP, dCMP & dTMP) with high accuracy [Clarke et al. 2009]. For example, aminocyclodextrin adaptor has been covalently attached within the α-hemolysin pore successfully. When a dNMP is captured and driven through the pore in a lipid bilayer membrane, the ionic current through the pore is reduced to one of four levels, each representing one of the four dNMP's (A, G, C, or T). Moreover, Robertson et al. [2007] have recently demonstrated that when a poly(ethylene glycol) (PEG) molecule enters a single α-hemolysin pore, it causes distinct mass-dependent conductance states with characteristic mean residence times. The conductance-based mass spectrum clearly resolves the repeat units of ethylene glycol, and the residence time increases with the mass of the PEG.

Although the current nanopore approach shows promise as a DNA detection method, the more demanding goal of accurate base-to-base sequencing has not yet been achieved.

SUMMARY OF THE INVENTION

A method for determining the nucleotide sequence of a single-stranded DNA comprising:

-   -   (a) contacting the single-stranded DNA, wherein the         single-stranded DNA is in an electrolyte solution in contact         with a nanopore in a membrane and wherein the single-stranded         DNA has a primer hybridized to a portion thereof, with a DNA         polymerase and four deoxyribonucleotide polyphosphate (dNPP)         analogues at least one of which can hybridize with each of an A,         T, G, or C nucleotide in the DNA being sequenced under         conditions permitting the DNA polymerase to catalyze         incorporation of one of the dNPP analogues into the primer if it         is complementary to the nucleotide residue of the         single-stranded DNA which is immediately 5′ to a nucleotide         residue of the single-stranded DNA hybridized to the 3′ terminal         nucleotide residue of the primer, so as to form a DNA extension         product, wherein each of the four dNPP analogues has the         structure:

-   -   -   wherein the base is adenine, guanine, cytosine, thymine or             uracil, or a derivative of one or more of these bases,             wherein R₁ is OH, wherein R₂ is H, wherein X is O, NH, S or             CH₂, wherein n is 1, 2, 3, or 4, wherein Z is O, S, or BH₃,             and with the proviso that (i) the type of base on each dNPP             analogue is different from the type of base on each of the             other three dNPP analogues, and (ii) either the value of n             of each dNPP analogue is different from the value of n of             each of the other three dNPP analogues, or the value of n of             each of the four dNPP analogues is the same and the type of             tag on each dNPP analogue is different from the type of tag             on each of the other three dNPP analogues, wherein             incorporation of the dNPP analogue results in release of a             polyphosphate having the tag attached thereto; and

    -   (b) identifying which dNPP analogue has been incorporated into         the primer to form a DNA extension product in step (a) by         applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from the         polyphosphate having the tag attached thereto generated in         step (a) translocating through the nanopore, wherein the         electronic change is different for each value of n, or for each         different type of tag, whichever is applicable, thereby         permitting identifying the nucleotide residue in the         single-stranded DNA complementary to the incorporated dNPP         analogue; and

    -   (c) repeatedly performing step (b) for each nucleotide residue         of the single-stranded DNA being sequenced, wherein in each         repetition of step (b) the dNPP analogue is incorporated into         the DNA extension product if it is complementary to the         nucleotide residue of the single-stranded DNA which is         immediately 5′ to a nucleotide residue of the single-stranded         DNA hybridized to the 3′ terminal nucleotide residue of the DNA         extension product,         thereby determining the nucleotide sequence of the         single-stranded DNA.

A method for determining the nucleotide sequence of a single-stranded DNA comprising:

-   -   (a) contacting the single-stranded DNA, wherein the         single-stranded DNA is in an electrolyte solution in contact         with a nanopore in a membrane and wherein the single-stranded         DNA has a primer hybridized to a portion thereof, a DNA         polymerase and a deoxyribonucleotide polyphosphate (dNPP)         analogue under conditions permitting the DNA polymerase to         catalyze incorporation of the dNPP analogue into the primer if         it is complementary to the nucleotide residue of the         single-stranded DNA which is immediately 5′ to a nucleotide         residue of the single-stranded DNA hybridized to the 3′ terminal         nucleotide residue of the primer, so as to form a DNA extension         product, wherein the dNPP analogue has the structure:

-   -   -   wherein the base is adenine, guanine, cytosine, uracil or             thymine, or a derivative of each thereof, wherein R₁ is —OH,             —O—CH₂N₃ or —O-2-nitrobenzyl, wherein R₂ is H, wherein X is             O, NH, S or CH₂, wherein n is 1, 2, 3, or 4, wherein Z is O,             S, or BH₃, and         -   wherein if the dNPP analogue is not incorporated,             iteratively repeating the contacting with a different dNPP             analogue until a dNPP analogue is incorporated, with the             proviso that (1) the type of base on each dNPP analogue is             different from the type of base on each of the other dNPP             analogues, and (2) either the value of n of each dNPP             analogue is different from the value of n of each of the             other three dNPP analogues, or the value of n of each of the             four dNPP analogues is the same and the type of tag on each             dNPP analogue is different from the type of tag on each of             the other three dNPP analogues,         -   wherein incorporation of a dNPP analogue results in release             of a polyphosphate having the tag attached thereto;

    -   (b) determining which dNPP analogue has been incorporated into         the primer to form a DNA extension product in step (a) by         applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from the         polyphosphate having the tag attached thereto generated in         step (a) translocating through the nanopore, wherein the         electronic change is different for each value of n, or for each         different type of tag, as appropriate, thereby identifying the         nucleotide residue in the single-stranded DNA complementary to         the incorporated dNPP analogue;

    -   (c) repeatedly performing steps (a) and (b) for each nucleotide         residue of the single-stranded DNA being sequenced, wherein in         each repetition of step (a) the dNPP analogue is incorporated         into the DNA extension product if it is complementary to the         nucleotide residue of the single-stranded DNA which is         immediately 5′ to a nucleotide residue of the single-stranded         DNA hybridized to the 3′ terminal nucleotide residue of the DNA         extension product,         thereby determining the nucleotide sequence of the         single-stranded DNA.

A method for determining the nucleotide sequence of a single-stranded DNA comprising:

-   -   (a) contacting the single-stranded DNA, wherein the         single-stranded DNA is in an electrolyte solution in contact         with a nanopore in a membrane and wherein the single-stranded         DNA has a primer hybridized to a portion thereof, with a DNA         polymerase and at least four deoxyribonucleotide polyphosphate         (dNPP) analogues under conditions permitting the DNA polymerase         to catalyze incorporation of one of the dNPP analogues into the         primer if it is complementary to the nucleotide residue of the         single-stranded DNA which is immediately 5′ to a nucleotide         residue of the single-stranded DNA hybridized to the 3′ terminal         nucleotide residue of the primer, so as to form a DNA extension         product, wherein each of the four dNPP analogues has a structure         chosen from the following:

-   -   -   wherein the base is adenine, guanine, cytosine, thymine or             uracil, or a derivative of each thereof, wherein Y is a tag,             wherein R₁, if present, is OH, wherein R₂, if present, is H,             wherein X is a cleavable linker, wherein Z is O, S or BH₃,             wherein n is 1, 2, 3, or 4, wherein A is O, S, CH₂, CHF,             CFF, or NH, and with the proviso that (i) the type of base             on each dNPP analogue is different from the type of base on             each of the other three dNPP analogues, and (ii) the type of             tag on each dNPP analogue is different from the type of tag             on each of the other three dNPP analogues;

    -   (b) cleaving the tag from the dNPP analogue incorporated in step         (a); and

    -   (c) determining which dNPP analogue was incorporated in step (a)         by applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from tag cleaved         off in step (b) translocating through the nanopore, wherein the         electronic change is different for each different type of tag,         thereby identifying the nucleotide residue in the         single-stranded DNA complementary to the incorporated dNPP         analogue; and

    -   (d) repeatedly performing steps (a), (b) and (c) for each         nucleotide residue of the single-stranded DNA being sequenced,         wherein in each repetition of step (a) the dNPP analogue is         incorporated into the DNA extension product resulting from the         previous iteration of step (a) if it is complementary to the         nucleotide residue of the single-stranded DNA which is         immediately 5′ to a nucleotide residue of the single-stranded         DNA hybridized to the 3′ terminal nucleotide residue of the DNA         extension product,         thereby determining the nucleotide sequence of the         single-stranded DNA.

A method for determining the nucleotide sequence of a single-stranded DNA comprising:

-   -   (a) contacting the single-stranded DNA, wherein the         single-stranded DNA is in an electrolyte solution in contact         with a nanopore in a membrane, wherein the single-stranded DNA         has a primer hybridized to a portion thereof, a DNA polymerase         and a deoxyribonucleotide polyphosphate (dNPP) analogue under         conditions permitting the DNA polymerase to catalyze         incorporation of the dNPP analogue into the primer if it is         complementary to the nucleotide residue of the single-stranded         DNA which is immediately 5′ to a nucleotide residue of the         single-stranded DNA hybridized to the 3′ terminal nucleotide         residue of the primer, so as to form a DNA extension product,         wherein the dNPP analogue has the structure:

-   -   -   wherein the base is adenine, guanine, cytosine, uracil or             thymine, or a derivative of each thereof, wherein Y is a             tag, and wherein R₁ if present is OH, —OCH₂N₃ or             —O-2-nitrobenzyl, R₂ if present is H, wherein X is a             cleavable linker, wherein Z is O, S or BH₃, wherein n is 1,             2, 3, or 4, wherein A is O, S, CH₂, CHF, CFF, or NH,         -   and if the dNPP analogue is not incorporated, iteratively             repeating the contacting with a different dNPP analogue             until a dNPP analogue is incorporated, with the proviso             that (1) the type of base on each dNPP analogue is different             from the type of base on each other dNPP analogue, and (2)             the type of tag on each dNPP analogue is different from the             type of tag on each other dNPP analogue, wherein             incorporation of a dNPP analogue results in release of a             polyphosphate having the tag attached thereto;

    -   (b) cleaving the tag from the dNPP analogue incorporated in step         (a); and

    -   (c) determining which dNPP analogue was incorporated in step (a)         to form a DNA extension product by applying a voltage across the         membrane and measuring an electronic change across the nanopore         resulting from the tag cleaved off in step (b) translocating         through the nanopore, wherein the electronic change is different         for each type of tag, thereby identifying the nucleotide residue         in the single-stranded DNA complementary to the incorporated         dNPP analogue;

    -   (d) iteratively performing steps (a) through (c) for each         nucleotide residue of the single-stranded DNA being sequenced,         wherein in each iteration of step (a) the dNPP analogue is         incorporated into the DNA extension product resulting from the         previous iteration of step (a) if it is complementary to the         nucleotide residue of the single-stranded DNA which is         immediately 5′ to a nucleotide residue of the single-stranded         DNA hybridized to the 3′ terminal nucleotide residue of the DNA         extension product,         thereby determining the nucleotide sequence of the         single-stranded DNA.

A process for producing a nucleotide triphosphate analogue, wherein the nucleotide triphosphate analogue differs from a nucleotide triphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         dicyclohexylcarbodiimide/dimethylformamide under conditions         permitting production of a cyclic trimetaphosphate;     -   b) contacting the product resulting from step a) with a tag         having a hydroxyl or amino group attached thereto under         conditions permitting nucleophilic opening of the cyclic         trimetaphosphate so as to bond the tag to a terminal phosphate         thereby forming the nucleotide triphosphate analogue.

A process for producing a nucleotide triphosphate analogue, wherein the nucleotide triphosphate analogue differs from a nucleotide triphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         dicyclohexylcarbodiimide/dimethylformamide under conditions         permitting production of a cyclic trimetaphosphate;     -   b) contacting the product resulting from step a) with a         nucleophile so as to form an —OH or —NH₂ functionalized         compound;     -   c) reacting the product of step b) with a tag having a —COR         group attached thereto under conditions permitting the tag to         bond indirectly to a terminal phosphate thereby forming the         nucleotide triphosphate analogue.

A process for producing a nucleotide tetraphosphate analogue, wherein the nucleotide tetraphosphate analogue differs from a nucleotide tetraphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a tag         having a monosphosphate group attached thereto under conditions         permitting formation of the nucleotide tetraphosphate analogue.

A process for producing a nucleotide tetraphosphate analogue, wherein the nucleotide tetraphosphate analogue differs from a nucleotide tetraphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with phosphoric         acid under conditions permitting formation of a nucleotide         tetraphosphate;

    -   c) contacting the nucleotide tetraphosphate with 1)         carbonyldiimidazole/dimethylformamide; 2) a nucleophile and         then 3) NH₄OH so as to form an —OH or —NH₂ functionalized         compound;

    -   d) contacting the product of step c) with a tag having a —COR         group attached thereto under conditions permitting the tag to         bond indirectly to a terminal phosphate thereby forming the         nucleotide tetraphosphate analogue.

A process for producing a nucleotide tetraphosphate analogue, wherein the nucleotide tetraphosphate analogue differs from a nucleotide tetraphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   b) contacting the product resulting from step a) with phosphoric         acid under conditions permitting formation of a nucleotide         tetraphosphate;     -   c) contacting the nucleotide tetraphosphate with         carbonyldiimidazole/dimethylformamide and a tag having a         hydroxyl or amino group attached thereto so as to form a         compound having the structure:

-   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is         adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or         a 5-methylpyrimidine.

A process for producing a nucleotide pentaphosphate analogue, wherein the nucleotide pentaphosphate analogue differs from a nucleotide pentaphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a tag         having a pyrophosphate group attached thereto under conditions         permitting formation of the nucleotide pentaphosphate analogue.

A process for producing a nucleotide pentaphosphate analogue, wherein the nucleotide pentaphosphate analogue differs from a nucleotide pentaphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₂ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a         pyrophosphate group under conditions permitting formation of a         nucleotide pentaphosphate;

    -   c) contacting the nucleotide pentaphosphate with         carbonyldiimidazole/dimethylformamide and a tag having a         hydroxyl or amino group attached thereto so as to form the         nucleotide pentaphosphate analogue.

A process for producing a nucleotide hexaphosphate analogue, wherein the nucleotide hexaphosphate analogue differs from a nucleotide hexaphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a tag         having a triphosphate group attached thereto under conditions         permitting formation of the nucleotide hexaphosphate analogue.

A process for producing a nucleotide hexaphosphate analogue, wherein the nucleotide hexaphosphate analogue differs from a nucleotide hexaphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a         triphosphate group under conditions permitting formation of a         nucleotide hexaphosphate;

    -   c) contacting the nucleotide hexaphosphate with         carbonyldiimidazole/dimethylformamide and a tag having a         hydroxyl or amino group attached thereto so as to form the         nucleotide hexaphosphate analogue.

A compound having the structure:

wherein the tag is ethylene glycol, an amino acid, a carbohydrate, a dye, mononucleotide, dinucleotide, trinucleotide, tetranucleotide, pentanucleotide or hexanucleotide, wherein R₁ is OH, wherein R₂ is H or OH, wherein X is O, NH, S or CH₂, wherein Z is O, S, or BH₃, wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine, and wherein n is 1, 2, 3, or 4.

A compound having the structure:

wherein in each structure n is, independently, 1, 2, 3 or 4, and m is, independently, an integer from 0 to 100, and wherein when m is 0 the terminal phosphate of the dNPP is bonded directly to the 3′ O atom of the nucleoside shown on the left hand side of the structure, wherein R₁ is —OH, or —O—CH₂N₃, and R₂ is H or OH.

A composition comprising at least four deoxynucleotide polyphosphate (dNPP) analogues, each having a structure selected from the structures set forth in claims 74 and 75, wherein each of the four dNPP analogues comprises a type of base different from the type of base of the other three dNPP analogues.

A compound having the structure:

wherein m an integer from 0 to 100, and wherein the compound comprises a single type of base, and wherein the base is adenine, guanine, cytosine, uracil or thymine or a derivative thereof of each.

A compound having the structure:

wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

A compound having the structure:

wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine, and R is a substituted or unsubstituted hydrocarbyl, up to 3000 daltons.

A compound having the structure:

A compound having the structure:

wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine, and m is an integer from 1-50.

A compound having the structure:

wherein n is 1 or 2 and the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

A compound having the structure:

wherein R₁ is —OH, or —O—CH₂N₃, and R₂ is H or OH.

A method for determining the nucleotide sequence of a single-stranded RNA comprising:

-   -   (a) contacting the single-stranded RNA, wherein the         single-stranded RNA is in an electrolyte solution in contact         with a nanopore in a membrane, wherein the single-stranded RNA         has a primer hybridized to a portion thereof, with a RNA         polymerase and at least four ribonucleotide polyphosphate (rNPP)         analogues under conditions permitting the RNA polymerase to         catalyze incorporation of one of the rNPP analogues into the         primer if it is complementary to the nucleotide residue of the         single-stranded RNA which is immediately 5′ to a nucleotide         residue of the single-stranded RNA hybridized to the 3′ terminal         nucleotide residue of the primer, so as to form a RNA extension         product, wherein each of the four rNPP analogues has the         structure:

-   -   -   wherein the base is adenine, guanine, cytosine, thymine or             uracil, or a derivative thereof of each, wherein R₁ is OH,             wherein R₂ is OH, wherein X is O, NH, S or CH₂, wherein n is             1, 2, 3, or 4, wherein Z is O, S, or BH₃, and with the             proviso that (i) the type of base on each rNPP analogue is             different from the type of base on each of the other three             rNPP analogues, and (ii) either the value of n of each rNPP             analogue is different from the value of n of each of the             other three rNPP analogues, or the value of n of each of the             four rNPP analogues is the same and the type of tag on each             rNPP analogue is different from the type of tag on each of             the other three rNPP analogues,         -   wherein incorporation of the rNPP analogue results in             release of a polyphosphate having the tag attached thereto;             and

    -   (b) determining which rNPP analogue has been incorporated into         the primer to form a RNA extension product in step (a) by         applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from the         polyphosphate having the tag attached thereto generated in         step (a) translocating through the nanopore, wherein the         electronic change is different for each value of n, or for each         different type of tag, as appropriate, thereby identifying the         nucleotide residue in the single-stranded RNA complementary to         the incorporated rNPP analogue; and

    -   (c) iteratively performing steps (a) and (b) for each nucleotide         residue of the single-stranded RNA being sequenced, wherein in         each iteration of step (a) the rNPP analogue is incorporated         into the RNA extension product resulting from the previous         iteration of step (a) if it is complementary to the nucleotide         residue of the single-stranded RNA which is immediately 5′ to a         nucleotide residue of the single-stranded RNA hybridized to the         3′ terminal         -   nucleotide residue of the RNA extension product, thereby             determining the nucleotide sequence of the single-stranded             RNA.

A method for determining the nucleotide sequence of a single-stranded RNA comprising:

-   -   (a) contacting the single-stranded RNA, wherein the         single-stranded RNA is in an electrolyte solution in contact         with a nanopore in a membrane and wherein the single-stranded         RNA has a primer hybridized to a portion thereof, a RNA         polymerase and a ribonucleotide polyphosphate (rNPP) analogue         under conditions permitting the RNA polymerase to catalyze         incorporation of the rNPP analogue into the primer if it is         complementary to the nucleotide residue of the single-stranded         RNA which is immediately 5′ to a nucleotide residue of the         single-stranded RNA hybridized to the 3′ terminal nucleotide         residue of the primer, so as to form a RNA extension product,         wherein the rNPP analogue has the structure:

-   -   -   wherein the base is adenine, guanine, cytosine, uracil or             thymine, wherein R₁ is —OH, —O—CH₂N₃ or —O-2-nitrobenzyl,             wherein R₂ is —OH, wherein X is O, NH, S or CH₂, wherein n             is 1, 2, 3, or 4, wherein Z is O, S, or BH₃,         -   and wherein if the rNPP analogue is not incorporated,             iteratively repeating the contacting with a different rNPP             analogue until a rNPP analogue is incorporated, with the             proviso that (1) the type of base on each rNPP analogue is             different from the type of base on each of the other rNPP             analogues, and (2) either the value of n of each rNPP             analogue is different from the value of n of each of the             other three rNPP analogues, or the value of n of each of the             four rNPP analogues is the same and the type of tag on each             rNPP analogue is different from the type of tag on each of             the other three rNPP analogues,         -   wherein incorporation of a rNPP analogue results in release             of a polyphosphate having the tag attached thereto;

    -   (b) determining which rNPP analogue has been incorporated into         the primer to form a RNA extension product in step (a) by         applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from the         polyphosphate having the tag attached thereto generated in         step (a) translocating through the nanopore, wherein the         electronic change is different for each value of n, or different         for each type of tag, as appropriate, thereby identifying the         nucleotide residue in the single-stranded RNA complementary to         the incorporated dNPP analogue;

    -   (c) iteratively performing steps (a) and (b) for each nucleotide         residue of the single-stranded RNA being sequenced, wherein in         each iteration of step (a) the rNPP analogue is incorporated         into the RNA extension product resulting from the previous         iteration of step (a) if it is complementary to the nucleotide         residue of the single-stranded RNA which is immediately 5′ to a         nucleotide residue of the single-stranded RNA hybridized to the         3′ terminal         -   nucleotide residue of the RNA extension product, thereby             determining the nucleotide sequence of the single-stranded             RNA.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 . α-Hemolysin protein self-assembles in a lipid bilayer to form an ion channel and a nucleic acid stretch passes through it (top), with the corresponding electronic signatures generated (bottom) [Vercoutere et al. 2001 and Deamer et al. 2002].

FIG. 2 . Structures of nucleotides deoxyribonucleotide adenosine triphosphate, deoxyribonucleotide guanosine triphosphate, deoxyribonucleotide cytosine triphosphate, and deoxyribonucleotide thymidine triphosphate.

FIG. 3 . Mechanism of primer extension and release of tagged-polyphosphate for detection.

FIG. 4 . Structure of four phosphate-tagged nucleoside-5′-polyphosphates.

FIG. 5 . Synthesis of phosphate-tagged nucleoside-5′-triphosphates.

FIG. 6 . Synthesis of phosphate-tagged nucleoside-5′-tetraphosphates.

FIG. 7 . Synthesis of terminal phosphate-tagged nucleoside-5′-pentaphosphates.

FIG. 8 . a) oligo-3′ to 5′-phosphate attachment, b) oligo-5′ to 5′-phosphate attachment, c) detectable moiety after polymerase reaction.

FIG. 9(A). Synthesis of base-modified nucleoside-5′-triphosphates. FIG. 9(B). Cleavage of base-modified nucleoside-5′-triphosphate and cleavage with TCEP.

FIG. 10 . Synthesis of 3′-O-modified nucleoside-5′-triphosphates. A. 3′-O-2-nitrobenzyl attached dNTPs; B. 3′-O-azidomethyl attached dNTPs; C. Detectable moiety after polymerase extension and TCEP cleavage; and D. Detectable moiety after polymerase extension and UV cleavage.

FIG. 11 . DNA extension reaction using phosphate modified nucleotide analogues.

FIG. 12 . DNA extension reaction using base-tagged nucleotide analogues.

FIG. 13 . DNA extension reaction using 2′- or 3′-OH labeled nucleotide analogues.

FIG. 14 . Schematic of DNA sequencing by nanopore with modified nucleotides, particularly applicable to single molecule real time sequencing involving addition of all 4 nucleotides and polymerase at same time to contact a single template molecule.

FIG. 15 . Phosphate, Base, 2′- and 3′-modified nucleoside phosphates with possible linkers and tags

-   -   BASE=adenine, guanine, thymine, cytosine, uracil, 5-methyl C,         7-deaza-A, 7-deaza-G or their derivatives thereof;     -   R₁ and R₂=H, OH, F, NH₂, N₃, or OR′;     -   n=1-5;     -   A=O, S, CH₂, CHF, CFF, NH;     -   Z═O, S, BH₃;     -   X=Linker which links phosphate or the 2′-O or 3′-O or the base         to the detectable moiety and may contain O, N or S, P atoms.         (The linker can also be a detectable moiety, directly or         indirectly, such as amino acids, peptides, proteins,         carbohydrates, PEGs of different length and molecular weights,         organic or inorganic dyes, fluorescent and fluorogenic dyes,         drugs, oligonucleotides, mass tags, chemiluminiscent tags and         may contain positive or negative charges.);     -   Y=tags or detectable moiety, such as aliphatic or organic         aromatic compounds with one or more rings, dyes, proteins,         carbohydrates, PEGs of different length and molecular weights,         drugs, oligonucleotides, mass tags, fluorescent tags,         chemiluminiscent tags and may contain positive or negative         charge.

FIG. 16 . Structures of PEG-phosphate-labeled nucleotides and examples of possible PEGs with different reactive groups to react with functional groups.

FIG. 17 . Non-limiting, specific examples of reactive groups on the terminal phosphates, which can also be attached with appropriate changes to a nucleoside base moiety, and groups with which groups can react to form tags.

FIG. 18 . A schematic of array of nanopores for massive parallel DNA sequencing by synthesis.

FIG. 19 . Synthesis of PEG-phosphate-labeled nucleotides.

FIG. 20 . MALDI-TOF mass spectra of the DNA extension products generated by incorporation of PEG-phosphate-labeled nucleotide analogues (dG4P-PEG). The single products shown in the spectra indicate that the dG4P-PEG24 and dG4P-PEG37 are incorporated at nearly 100% efficiency.

FIG. 21 . The relative blockade depth distributions for α-hemolysin nanopore in the presence of PEGs that contain either 49, 37, 24, or 16 ethylene oxide monomers at +40 mV applied potential. The four species are easily identified.

FIG. 22 . (A) Separation and mass distribution of mixed poly (ethylene glycol) (PEG) units through a single nanopore; and (B) selection of 4 distinct PEG units with base line separation as tags for the 4 bases, A, C, G, and T. The structures of linear and branched PEGs are also shown.

FIG. 23 . Synthesis of charged PEG-triphosphates (the charge can be adjusted based on the requirements).

FIG. 24 . Synthesis of phosphate-tagged nucleoside-5′-triphosphates.

FIG. 25 . Synthesis of phosphate-tagged nucleoside-5′-tetraphosphates

FIG. 26 . Synthesis of terminal phosphate-tagged nucleoside-5′-pentaphosphates.

FIG. 27 . CMOS-integrated nanopore measurement platform: (A) a micrograph of the eight-channel CMOS preamplifier chip with an image of one amplifier channel with the integrated cis-side electrode; (B) diagram showing the two-chip integration with a solid-state nanopore; (C) diagram showing the cross section of the chip and how the nanopore is etched directly into the chip in the one-chip implementation; packaging occurs with an independent well on the cis side; and a TEM image of a 3.5-nm-diameter nanopore.

FIG. 28 . Electrical performance of the CMOS-integrated nanopore electronics (A) Input-referred baseline current noise spectrum for C_(F)=0.15 pF, 1 MHz 4-pole Bessel filter, f_(s)=4MS/s. Also shown is the measured open-headstage of an Axopatch 200B in whole-cell mode with β=1, 100 kHz 4-pole Bessel filter, f_(s)=250kS/s. (B) Noise floor of the new amplifier with a nanopore attached compared with the same nanopore measured by the Axopatch 200B.

FIG. 29 . Tethering of the polymerase in the vicinity of the nanopore. A well helps to restrict diffusion. L denotes the critical distance from the pore opening at which molecular motions due to diffusion and electrophoresis are equal.

FIG. 30 . Synthesis of Tag-labeled-nucleoside-5′-polyphosphates.

FIG. 31 . Synthesis of 3′-O-blocked-PEG-nucleotides.

FIG. 32 . Sequencing by synthesis with PEG-nucleotides and nanopore detection (many copies of the same DNA molecule immobilized on a bead and addition of one PEG-nucleotide at a time). Use same PEGattached to the all four nucleotides. Add one PEG-nucleotide at a time, reads at least one base per cycle if correct nucleotide is incorporated.

FIG. 33 . Sequencing by synthesis with 3′-O-blocked-PEG-nucleotides and nanopore detection (many copies of same DNA molecule immobilized on a bead and addition of all four 3′-O-blocked-PEG-nucleotides). Add all four 3′-blocked, different size PEG attached nucleotides (3′=blocked dNTP-PEGs) together. Detection of the incorporated nucleotide based on the blockade signal of the released PEGs. The 3′-blocking group is removed by TECP treatment and continue cycle for correctly sequence the template including homopolymeric regions.

FIG. 34 . A schematic for a massive parallel way high density array of micro wells to perform the biochemical process. Each well can hold a different DNA template and nanopore device.

DETAILED DESCRIPTION OF THE INVENTION

A method for determining the nucleotide sequence of a single-stranded DNA comprising:

-   -   (a) contacting the single-stranded DNA, wherein the         single-stranded DNA is in an electrolyte solution in contact         with a nanopore in a membrane and wherein the single-stranded         DNA has a primer hybridized to a portion thereof, with a DNA         polymerase and at least four deoxyribonucleotide polyphosphate         (dNPP) analogues under conditions permitting the DNA polymerase         to catalyze incorporation of one of the dNPP analogues into the         primer if it is complementary to the nucleotide residue of the         single-stranded DNA which is immediately 5′ to a nucleotide         residue of the single-stranded DNA hybridized to the 3′ terminal         nucleotide residue of the primer, so as to form a DNA extension         product, wherein each of the four dNPP analogues has the         structure:

-   -   -   wherein the base is adenine, guanine, cytosine, thymine or             uracil, or a derivative of each thereof, wherein R₁ is OH,             wherein R₂ is H, wherein X is O, NH, S or CH₂, wherein n is             1, 2, 3, or 4, wherein Z is O, S, or BH₃, and with the             proviso that (i) the type of base on each dNPP analogue is             different from the type of base on each of the other three             dNPP analogues, and (ii) either the value of n of each dNPP             analogue is different from the value of n of each of the             other three dNPP analogues, or the value of n of each of the             four dNPP analogues is the same and the type of tag on each             dNPP analogue is different from the type of tag on each of             the other three dNPP analogues,         -   wherein incorporation of the dNPP analogue results in             release of a polyphosphate having the tag attached thereto;             and

    -   (b) determining which dNPP analogue has been incorporated into         the primer to form a DNA extension product in step (a) by         applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from the         polyphosphate having the tag attached thereto generated in         step (a) translocating through the nanopore, wherein the         electronic change is different for each value of n, or for each         different type of tag, as appropriate, thereby identifying the         nucleotide residue in the single-stranded DNA complementary to         the incorporated dNPP analogue; and

    -   (c) iteratively performing steps (a) and (b) for each nucleotide         residue of the single-stranded DNA being sequenced, wherein in         each iteration of step (a) the dNPP analogue is incorporated         into the DNA extension product resulting from the previous         iteration of step (a) if it is complementary to the nucleotide         residue of the single-stranded DNA which is immediately 5′ to a         nucleotide residue of the single-stranded DNA hybridized to the         3′ terminal         -   nucleotide residue of the DNA extension product, thereby             determining the nucleotide sequence of the single-stranded             DNA.

A method for determining the nucleotide sequence of a single-stranded DNA comprising:

-   -   (a) contacting the single-stranded DNA, wherein the         single-stranded DNA is in an electrolyte solution in contact         with a nanopore in a membrane and wherein the single-stranded         DNA has a primer hybridized to a portion thereof, a DNA         polymerase and a deoxyribonucleotide polyphosphate (dNPP)         analogue under conditions permitting the DNA polymerase to         catalyze incorporation of the dNPP analogue into the primer if         it is complementary to the nucleotide residue of the         single-stranded DNA which is immediately 5′ to a nucleotide         residue of the single-stranded DNA hybridized to the 3′ terminal         nucleotide residue of the primer, so as to form a DNA extension         product, wherein the dNPP analogue has the structure:

-   -   -   wherein the base is adenine, guanine, cytosine, uracil or             thymine, or a derivative of each thereof, wherein R₁ is —OH,             —O—CH₂N₃ or —O-2-nitrobenzyl, wherein R₂ is H, wherein X is             O, NH, S or CH₂, wherein n is 1, 2, 3, or 4, wherein Z is O,             S, or BH₃,         -   and wherein if the dNPP analogue is not incorporated,             iteratively repeating the contacting with a different dNPP             analogue until a dNPP analogue is incorporated, with the             proviso that (1) the type of base on each dNPP analogue is             different from the type of base on each of the other dNPP             analogues, and (2) either the value of n of each dNPP             analogue is different from the value of n of each of the             other three dNPP analogues, or the value of n of each of the             four dNPP analogues is the same and the type of tag on each             dNPP analogue is different from the type of tag on each of             the other three dNPP analogues,         -   wherein incorporation of a dNPP analogue results in release             of a polyphosphate having the tag attached thereto;

    -   (b) determining which dNPP analogue has been incorporated into         the primer to form a DNA extension product in step (a) by         applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from the         polyphosphate having the tag attached thereto generated in         step (a) translocating through the nanopore, wherein the         electronic change is different for each value of n, or for each         different type of tag, as appropriate, thereby identifying the         nucleotide residue in the single-stranded DNA complementary to         the incorporated dNPP analogue;

    -   (c) iteratively performing steps (a) and (b) for each nucleotide         residue of the single-stranded DNA being sequenced, wherein in         each iteration of step (a) the dNPP analogue is incorporated         into the DNA extension product resulting from the previous         iteration of step (a) if it is complementary to the nucleotide         residue of the single-stranded DNA which is immediately 5′ to a         nucleotide residue of the single-stranded DNA hybridized to the         3′ terminal         -   nucleotide residue of the DNA extension product, thereby             determining the nucleotide sequence of the single-stranded             DNA.

A method for determining the nucleotide sequence of a single-stranded DNA comprising:

-   -   (a) contacting the single-stranded DNA, wherein the         single-stranded DNA is in an electrolyte solution in contact         with a nanopore in a membrane and wherein the single-stranded         DNA has a primer hybridized to a portion thereof, with a DNA         polymerase and at least four deoxyribonucleotide polyphosphate         (dNPP) analogues under conditions permitting the DNA polymerase         to catalyze incorporation of one of the dNPP analogues into the         primer if it is complementary to the nucleotide residue of the         single-stranded DNA which is immediately 5′ to a nucleotide         residue of the single-stranded DNA hybridized to the 3′ terminal         nucleotide residue of the primer, so as to form a DNA extension         product, wherein each of the four dNPP analogues has a structure         chosen from the following:

-   -   -   wherein the base is adenine, guanine, cytosine, uracil or             thymine, or a derivative of each thereof, wherein Y is a             tag, wherein R₁, if present, is OH, wherein R₂, if present,             is H, wherein X is a cleavable linker, wherein Z is O, S or             BH₃, wherein n is 1, 2, 3, or 4, wherein A is O, S, CH₂,             CHF, CFF, or NH, and with the proviso that (i) the type of             base on each dNPP analogue is different from the type of             base on each of the other three dNPP analogues, and (ii) the             type of tag on each dNPP analogue is different from the type             of tag on each of the other three dNPP analogues;

    -   (b) cleaving the tag from the dNPP analogue incorporated in step         (a); and

    -   (c) determining which dNPP analogue was incorporated in step (a)         by applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from tag cleaved         off in step (b) translocating through the nanopore, wherein the         electronic change is different for each different type of tag,         thereby identifying the nucleotide residue in the         single-stranded DNA complementary to the incorporated dNPP         analogue; and

    -   (d) iteratively performing steps (a), (b) and (c) for each         nucleotide residue of the single-stranded DNA being sequenced,         wherein in each iteration of step (a) the dNPP analogue is         incorporated into the DNA extension product resulting from the         previous iteration of step (a) if it is complementary to the         nucleotide residue of the single-stranded DNA which is         immediately 5′ to a nucleotide residue of the single-stranded         DNA hybridized to the 3′ terminal         -   nucleotide residue of the DNA extension product, thereby             determining the nucleotide sequence of the single-stranded             DNA.

A method for determining the nucleotide sequence of a single-stranded DNA comprising:

-   -   (a) contacting the single-stranded DNA, wherein the         single-stranded DNA is in an electrolyte solution in contact         with a nanopore in a membrane, wherein the single-stranded DNA         has a primer hybridized to a portion thereof, a DNA polymerase         and a deoxyribonucleotide polyphosphate (dNPP) analogue under         conditions permitting the DNA polymerase to catalyze         incorporation of the dNPP analogue into the primer if it is         complementary to the nucleotide residue of the single-stranded         DNA which is immediately 5′ to a nucleotide residue of the         single-stranded DNA hybridized to the 3′ terminal nucleotide         residue of the primer, so as to form a DNA extension product,         wherein the dNPP analogue has the structure:

-   -   -   wherein the base is adenine, guanine, cytosine, uracil or             thymine, or derivative of each thereof, wherein Y is a tag,             and wherein R₁ if present is OH, —OCH₂N₃ or             —O-2-nitrobenzyl, R₂ if present is H, wherein X is a             cleavable linker, wherein Z is O, S or BH₃, wherein n is 1,             2, 3, or 4, wherein A is O, S, CH₂, CHF, CFF, or NH,         -   and if the dNPP analogue is not incorporated, iteratively             repeating the contacting with a different dNPP analogue             until a dNPP analogue is incorporated, with the proviso             that (1) the type of base on each dNPP analogue is different             from the type of base on each other dNPP analogue, and (2)             the type of tag on each dNPP analogue is different from the             type of tag on each other dNPP analogue, wherein             incorporation of a dNPP analogue results in release of a             polyphosphate having the tag attached thereto;

    -   (b) cleaving the tag from the dNPP analogue incorporated in step         (a); and

    -   (c) determining which dNPP analogue was incorporated in step (a)         to form a DNA extension product by applying a voltage across the         membrane and measuring an electronic change across the nanopore         resulting from the tag cleaved off in step (b) translocating         through the nanopore, wherein the electronic change is different         for each type of tag, thereby identifying the nucleotide residue         in the single-stranded DNA complementary to the incorporated         dNPP analogue;

    -   (d) iteratively performing steps (a) through (c) for each         nucleotide residue of the single-stranded DNA being sequenced,         wherein in each iteration of step (a) the dNPP analogue is         incorporated into the DNA extension product resulting from the         previous iteration of step (a) if it is complementary to the         nucleotide residue of the single-stranded DNA which is         immediately 5′ to a nucleotide residue of the single-stranded         DNA hybridized to the 3′ terminal         -   nucleotide residue of the DNA extension product, thereby             determining the nucleotide sequence of the single-stranded             DNA.

In an embodiment of the methods the tag is ethylene glycol, an amino acid, a carbohydrate, a dye, a mononucleotide, a dinucleotide, a trinucleotide, a tetranucleotide, a pentanucleotide or a hexanucleotide, a fluorescent dyes, a chemiluminiscent compound, an amino acid, a peptide, a carbohydrate, a nucleotide monophopshate, a nucleotide diphosphate, an aliphatic acid or an aromatic acid or an alcohol or a thiol with unsubstituted or substituted with one or more halogens, a cyano group, a nitro group, an alkyl group, an alkenyl group, an alkynyl group, an azido group.

In an embodiment of the methods the base is selected from the group consisting of adenine, guanine, cytosine, thymine, 7-deazaguanine, 7-deazaadenine or 5-methylcytosine.

In an embodiment the methods further comprise a washing step after each iteration of step (b) to remove unincoporated dNPP analogues from contact with the single-stranded DNA.

In an embodiment the methods further comprise a washing step after each iteration of step (c) to remove unincoporated dNPP analogues from contact with the single-stranded DNA.

In an embodiment the methods further comprise wherein the single-stranded DNA, electrolyte solution and nanopore in the membrane are located within a single container.

In an embodiment of the methods wherein R₁ is —O—CH₂N₃, the methods optionally further comprise treating the incorporated dNPP analogue so as to remove the —CH₂N₃ and result in an OH group attached to the 3′ position thereby permitting incorporation of a further dNPP analogue.

In an embodiment of the methods wherein R₁ is —O-2-nitrobenzyl, the methods optionally further comprise treating the incorporated nucleotide analogue so as to remove the −2-nitrobenzyl and result in an OH group attached to the 3′ position thereby permitting incorporation of a further dNPP analogue.

In an embodiment of the methods the dNPP analogues have the following structures:

wherein R₁ is OH, wherein R₂ is H or OH, wherein Z is O, S, or BH₃, and wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

In an embodiment of the methods the tag is a mononucleotide, a dinucleotide, a trinucleotide, a tetranucleotide, a pentanucleotide or a hexanucleotide and wherein the base of the mononucleotide, the dinucleotide, the trinucleotide, the tetranucleotide, the pentanucleotide or the hexanucleotide is the same type of base as the base of the dNPP analogue.

In an embodiment of the methods the tag is chosen from the following:

wherein in each structure n is, independently, 1, 2, 3 or 4, and m is, independently, an integer from 0 to 100, and wherein when m is 0 the terminal phosphate of the dNPP is bonded directly to the 3′ O atom of the nucleoside shown on the left hand side of the structure, and wherein the value of n is different for each type of base.

In an embodiment of the methods m is an integer from 0 to 50.

In an embodiment of the methods m is an integer from 0 to 10.

In an embodiment of the methods the dNPP analogue has the structure:

wherein R is a substituted or unsubstituted hydrocarbyl, up to 3000 daltons, and wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

In an embodiment of the methods the dNPP analogue has the structure:

wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

In an embodiment of the methods the dNPP analogue has the structure:

In an embodiment of the methods the dNPP analogue has the structure:

wherein m is an integer from 1-50, and wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

In an embodiment of the methods the electronic change is a change in current amplitude.

In an embodiment of the methods the electronic change is a change in conductance of the nanopore.

In an embodiment of the methods the nanopore is biological. In an embodiment of the methods the nanopore is proteinaceous. In an embodiment of the methods the nanopore comprises alpha hemolysin. In an embodiment of the methods the nanopore is graphene. In an embodiment of the methods the nanopore is a solid-state nanopore. In an embodiment of the methods the nanopore is in a solid-state membrane.

In an embodiment of the methods the single stranded DNA, the primer, or the DNA polymerase is attached to a solid surface.

In another embodiment of the methods the nanopore is part of an array of nanopores.

A process for producing a nucleotide triphosphate analogue, wherein the nucleotide triphosphate analogue differs from a nucleotide triphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         dicyclohexylcarbodiimide/dimethylformamide under conditions         permitting production of a cyclic trimetaphosphate;     -   b) contacting the product resulting from step a) with a tag         having a hydroxyl or amino group attached thereto under         conditions permitting nucleophilic opening of the cyclic         trimetaphosphate so as to bond the tag to a terminal phosphate         thereby forming the nucleotide triphosphate analogue.

A process for producing a nucleotide triphosphate analogue, wherein the nucleotide triphosphate analogue differs from a nucleotide triphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         dicyclohexylcarbodiimide/dimethylformamide under conditions         permitting production of a cyclic trimetaphosphate;     -   b) contacting the product resulting from step a) with a         nucleophile so as to form an —OH or —NH₂ functionalized         compound;     -   c) reacting the product of step b) with a tag having a —COR         group attached thereto under conditions permitting the tag to         bond indirectly to a terminal phosphate thereby forming the         nucleotide triphosphate analogue.

In an embodiment of the instant process the nucleophile is H₂N—R—OH, H₂N—R—NH₂, R′S—R—OH, R′S—R—NH₂, or

In an embodiment the instant process comprises in step b) contacting the product resulting from step a) with a compound having the structure:

and then NH₄OH so as to form a compound having the structure:

and reacting the product of step b) with a tag having a —COR group attached thereto under conditions permitting the tag to bond indirectly to a terminal phosphate thereby forming the nucleotide triphosphate analogue having the structure:

wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

A process for producing a nucleotide tetraphosphate analogue, wherein the nucleotide tetraphosphate analogue differs from a nucleotide tetraphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a tag         having a monosphosphate group attached thereto under conditions         permitting formation of the nucleotide tetraphosphate analogue.

A process for producing a nucleotide tetraphosphate analogue, wherein the nucleotide tetraphosphate analogue differs from a nucleotide tetraphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₄ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with phosphoric         acid under conditions permitting formation of a nucleotide         tetraphosphate;

    -   c) contacting the nucleotide tetraphosphate with 1)         carbonyldiimidazole/dimethylformamide; 2) a nucleophile and         then 3) NH₄OH so as to form an —OH or —NH₂ functionalized         compound;

    -   d) contacting the product of step c) with a tag having a —COR         group attached thereto under conditions permitting the tag to         bond indirectly to a terminal phosphate thereby forming the         nucleotide tetraphosphate analogue.

In an embodiment of the instant process the nucleophile is H₂N—R—OH, H₂N—R—NH₂, R′S—R—OH, R′S—R—NH₂, or

In an embodiment the instant process comprises in step b) contacting the nucleotide tetraphosphate with 1) carbonyldiimidazole/dimethylformamide; 2) a compound having the structure:

and then 3) NH₄OH so as to form a compound having the structure:

and contacting the product of step b) with a tag having a —COR group attached thereto under conditions permitting the tag to bond indirectly to a terminal phosphate thereby forming the nucleotide triphosphate analogue having the structure:

wherein R₄ is OH, wherein R₂ is H or OH, wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

A process for producing a nucleotide tetraphosphate analogue, wherein the nucleotide tetraphosphate analogue differs from a nucleotide tetraphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   b) contacting the product resulting from step a) with phosphoric         acid under conditions permitting formation of a nucleotide         tetraphosphate;     -   c) contacting the nucleotide tetraphosphate with         carbonyldiimidazole/dimethylformamide and a tag having a         hydroxyl or amino group attached thereto so as to form a         compound having the structure:

-   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is         adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or         a 5-methylpyrimidine.

A process for producing a nucleotide pentaphosphate analogue, wherein the nucleotide pentaphosphate analogue differs from a nucleotide pentaphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a tag         having a pyrophosphate group attached thereto under conditions         permitting formation of the nucleotide pentaphosphate analogue.

A process for producing a nucleotide pentaphosphate analogue, wherein the nucleotide pentaphosphate analogue differs from a nucleotide pentaphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₂ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a         pyrophosphate group under conditions permitting formation of a         nucleotide pentaphosphate;

    -   c) contacting the nucleotide pentaphosphate with         carbonyldiimidazole/dimethylformamide and a tag having a         hydroxyl or amino group attached thereto so as to form the         nucleotide pentaphosphate analogue.

A process for producing a nucleotide hexaphosphate analogue, wherein the nucleotide hexaphosphate analogue differs from a nucleotide hexaphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a tag         having a triphosphate group attached thereto under conditions         permitting formation of the nucleotide hexaphosphate analogue.

A process for producing a nucleotide hexaphosphate analogue, wherein the nucleotide hexaphosphate analogue differs from a nucleotide hexaphosphate by having a tag attached to the terminal phosphate thereof, comprising:

-   -   a) contacting a nucleotide triphosphate with         1,1′-carbonyldiimidazole/dimethylformamide under conditions         permitting formation of the following structure:

-   -   -   wherein R₁ is OH, wherein R₂ is H or OH, wherein the base is             adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine             or a 5-methylpyrimidine;

    -   b) contacting the product resulting from step a) with a         triphosphate group under conditions permitting formation of a         nucleotide hexaphosphate;

    -   c) contacting the nucleotide hexaphosphate with         carbonyldiimidazole/dimethylformamide and a tag having a         hydroxyl or amino group attached thereto so as to form the         nucleotide hexaphosphate analogue.

A compound having the structure:

-   -   wherein the tag is ethylene glycol, an amino acid, a         carbohydrate, a dye, mononucleotide, dinucleotide,         trinucleotide, tetranucleotide, pentanucleotide or         hexanucleotide, wherein R₁ is OH, wherein R₂ is H or OH, wherein         X is O, NH, S or CH₂, wherein Z is O, S, or BH₃, wherein the         base is adenine, guanine, cytosine, thymine, uracil, a         7-deazapurine or a 5-methylpyrimidine, and wherein n is 1, 2, 3,         or 4.

In an embodiment R₂ is H. In an embodiment R₂ is OH.

A compound having the structure:

wherein in each structure n is, independently, 1, 2, 3 or 4, and m is, independently, an integer from 0 to 100, and wherein when m is 0 the terminal phosphate of the dNTP is bonded directly to the 3′ O atom of the nucleoside shown on the left hand side of the structure, wherein R₁ is —OH, or —O—CH₂N₃, and R₂ is H or OH.

In an embodiment m is from 0 to 50. In an embodiment m is from 0 to 10. In an embodiment R₁ is —OH. In an embodiment R₂ is —H. In an embodiment R₂ is —OH.

A compound having the structure:

wherein m an integer from 0 to 100, and wherein the compound comprises a single type of base, and wherein the base is adenine, guanine, cytosine, uracil or thymine or a derivative thereof of each.

In an embodiment m is from 0 to 50. In an embodiment m is from 0 to 10.

In an embodiment the compound has the structure:

-   -   wherein m is an integer from 0 to 100.

A compound having the structure:

wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

A compound having the structure:

wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine, and R is a substituted or unsubstituted hydrocarbyl, up to 3000 daltons.

A compound having the structure:

A compound having the structure:

wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine, and m is an integer from 1-50.

A compound having the structure:

wherein n is 1 or 2 and the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

A compound having the structure:

wherein R₁ is —OH, or —O—CH₂N₃, and R₂ is H or OH.

A method for determining the nucleotide sequence of a single-stranded RNA comprising:

-   -   (a) contacting the single-stranded RNA, wherein the         single-stranded RNA is in an electrolyte solution in contact         with a nanopore in a membrane, wherein the single-stranded RNA         has a primer hybridized to a portion thereof, with a RNA         polymerase and at least four ribonucleotide polyphosphate (rNPP)         analogues under conditions permitting the RNA polymerase to         catalyze incorporation of one of the rNPP analogues into the         primer if it is complementary to the nucleotide residue of the         single-stranded RNA which is immediately 5′ to a nucleotide         residue of the single-stranded RNA hybridized to the 3′ terminal         nucleotide residue of the primer, so as to form a RNA extension         product, wherein each of the four rNPP analogues has the         structure:

-   -   -   wherein the base is adenine, guanine, cytosine, thymine or             uracil, or a derivative thereof of each, wherein R₁ is OH,             wherein R₂ is OH, wherein X is O, NH, S or CH₂, wherein n is             1, 2, 3, or 4, wherein Z is O, S, or BH₃, and with the             proviso that (i) the type of base on each rNPP analogue is             different from the type of base on each of the other three             rNPP analogues, and (ii) either the value of n of each rNPP             analogue is different from the value of n of each of the             other three rNPP analogues, or the value of n of each of the             four rNPP analogues is the same and the type of tag on each             rNPP analogue is different from the type of tag on each of             the other three rNPP analogues,         -   wherein incorporation of the rNPP analogue results in             release of a polyphosphate having the tag attached thereto;             and

    -   (b) determining which rNPP analogue has been incorporated into         the primer to form a RNA extension product in step (a) by         applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from the         polyphosphate having the tag attached thereto generated in         step (a) translocating through the nanopore, wherein the         electronic change is different for each value of n, or for each         different type of tag, as appropriate, thereby identifying the         nucleotide residue in the single-stranded RNA complementary to         the incorporated rNPP analogue; and

    -   (c) iteratively performing steps (a) and (b) for each nucleotide         residue of the single-stranded RNA being sequenced, wherein in         each iteration of step (a) the rNPP analogue is incorporated         into the RNA extension product resulting from the previous         iteration of step (a) if it is complementary to the nucleotide         residue of the single-stranded RNA which is immediately 5′ to a         nucleotide residue of the single-stranded RNA hybridized to the         3′ terminal         -   nucleotide residue of the RNA extension product, thereby             determining the nucleotide sequence of the single-stranded             RNA.

A method for determining the nucleotide sequence of a single-stranded RNA comprising:

-   -   (a) contacting the single-stranded RNA, wherein the         single-stranded RNA is in an electrolyte solution in contact         with a nanopore in a membrane and wherein the single-stranded         RNA has a primer hybridized to a portion thereof, a RNA         polymerase and a ribonucleotide polyphosphate (rNPP) analogue         under conditions permitting the RNA polymerase to catalyze         incorporation of the rNPP analogue into the primer if it is         complementary to the nucleotide residue of the single-stranded         RNA which is immediately 5′ to a nucleotide residue of the         single-stranded RNA hybridized to the 3′ terminal nucleotide         residue of the primer, so as to form a RNA extension product,         wherein the rNPP analogue has the structure:

-   -   -   wherein the base is adenine, guanine, cytosine, uracil or             thymine, wherein R₁ is —OH, —O—CH₂N₃ or —O-2-nitrobenzyl,             wherein R₂ is —OH, wherein X is O, NH, S or CH₂, wherein n             is 1, 2, 3, or 4, wherein Z is O, S, or BH₃,         -   and wherein if the rNPP analogue is not incorporated,             iteratively repeating the contacting with a different rNPP             analogue until a rNPP analogue is incorporated, with the             proviso that (1) the type of base on each rNPP analogue is             different from the type of base on each of the other rNPP             analogues, and (2) either the value of n of each rNPP             analogue is different from the value of n of each of the             other three rNPP analogues, or the value of n of each of the             four rNPP analogues is the same and the type of tag on each             rNPP analogue is different from the type of tag on each of             the other three rNPP analogues,         -   wherein incorporation of a rNPP analogue results in release             of a polyphosphate having the tag attached thereto;

    -   (b) determining which rNPP analogue has been incorporated into         the primer to form a RNA extension product in step (a) by         applying a voltage across the membrane and measuring an         electronic change across the nanopore resulting from the         polyphosphate having the tag attached thereto generated in         step (a) translocating through the nanopore, wherein the         electronic change is different for each value of n, or different         for each type of tag, as appropriate, thereby identifying the         nucleotide residue in the single-stranded RNA complementary to         the incorporated dNPP analogue;

    -   (c) iteratively performing steps (a) and (b) for each nucleotide         residue of the single-stranded RNA being sequenced, wherein in         each iteration of step (a) the rNPP analogue is incorporated         into the RNA extension product resulting from the previous         iteration of step (a) if it is complementary to the nucleotide         residue of the single-stranded RNA which is immediately 5′ to a         nucleotide residue of the single-stranded RNA hybridized to the         3′ terminal         -   nucleotide residue of the RNA extension product, thereby             determining the nucleotide sequence of the single-stranded             RNA.

In an embodiment the dNPP analogue has the structure:

wherein n is 1 or 2 and the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine.

In an embodiment the biological nanopore is integrated with CMOS electronics. In another embodiment the solid-state nanopore is integrated with CMOS electronics.

In an embodiment the attachment to the solid surface is via biotin-streptavidin linkages. In another embodiment the DNA polymerase is attached to the solid surface via gold surface modified with an alkanethiol self-assembled monolayer functionalized with amino groups, wherein the amino groups are modified to NHS esters for attachment to amino groups on the DNA polymerase.

In one embodiment the dNPP analogue is a terminal-phosphate-tagged nucleoside-polyphosphate. In a further embodiment each type of dNPP analogue has a polyethylene glycol tag which differs in size from the polyethylene glycol tags of each of the other three types of dNPP analogues.

In one embodiment the tag has the structure as follows:

wherein W is an integer between 0 and 100.

In another embodiment the tag has the structure as follows:

wherein R is NH₂, OH, COOH, CHO, SH, or N₃, and W is an integer from 0 to 100.

A composition comprising at least four deoxynucleotide polyphosphate (dNPP) analogues, each having a structure selected from the structures set forth in claims 74 and 75, wherein each of the four dNPP analogues comprises a type of base different from the type of base of the other three dNPP analogues.

In one embodiment, each of the four dNPP analogues has a polyethylene glycol tag which is different in size from the polyetheylene glycol tags of each of the other three dNPP analogues.

In an embodiment net charge on the tagged nucleoside polyphosphate is neutral. In another embodiment the released tag has a positive charge.

In one embodiment, the method further comprising a step of treating with alkaline phosphatase after step b), wherein the alkaline phosphatase hydrolyzes free phosphate groups on the released tag-pyrophosphate.

In one embodiment multiple copies of the single-stranded DNA are immobilized on a bead.

A “derivative” of adenine, guanine, cytosine, thymine or uracil, include a 7-deaza-purine and a 5-methyl pyrimidine. Examples include 7-deaza adenine, 7-deaza-guanine, and 5-methyl-cytosine.

The present invention also provides a compound having the structure of any of the compounds set forth in the figures and/or schemes of the present application.

The present invention also provides a dNPP analogue comprising a tag having the structure of any of the tags set forth in the figures and/or schemes of the present application.

In an embodiment, the tag is a hydrocarbyl, substituted or unsubstituted, such as an alkyl, akenyl, alkynyl, and having a mass of 3000 daltons or less.

As used herein, “alkyl” includes both branched and straight-chain saturated aliphatic hydrocarbon groups having the specified number of carbon atoms and may be unsubstituted or substituted. Thus, C1-Cn as in “C1-Cn alkyl” is defined to include groups having 1, 2, . . . , n−1 or n carbons in a linear or branched arrangement. For example, a “C1-05 alkyl” is defined to include groups having 1, 2, 3, 4, or 5 carbons in a linear or branched arrangement, and specifically includes methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, and pentyl

As used herein, “alkenyl” refers to a non-aromatic hydrocarbon radical, straight or branched, containing at least 1 carbon to carbon double bond, and up to the maximum possible number of non-aromatic carbon-carbon double bonds may be present, and may be unsubstituted or substituted. For example, “C2-C5 alkenyl” means an alkenyl radical having 2, 3, 4, or 5, carbon atoms, and up to 1, 2, 3, or 4, carbon-carbon double bonds respectively. Alkenyl groups include ethenyl, propenyl, and butenyl.

The term “alkynyl” refers to a hydrocarbon radical straight or branched, containing at least 1 carbon to carbon triple bond, and up to the maximum possible number of non-aromatic carbon-carbon triple bonds may be present, and may be unsubstituted or substituted. Thus, “C2-C5 alkynyl” means an alkynyl radical having 2 or 3 carbon atoms and 1 carbon-carbon triple bond, or having 4 or 5 carbon atoms and up to 2 carbon-carbon triple bonds. Alkynyl groups include ethynyl, propynyl and butynyl.

The term “substituted” refers to a functional group as described above such as an alkyl, or a hydrocarbyl, in which at least one bond to a hydrogen atom contained therein is replaced by a bond to non-hydrogen or non-carbon atom, provided that normal valencies are maintained and that the substitution(s) result(s) in a stable compound. Substituted groups also include groups in which one or more bonds to a carbon(s) or hydrogen(s) atom are replaced by one or more bonds, including double or triple bonds, to a heteroatom. Non-limiting examples of substituents include the functional groups described above, and for example, N, e.g. so as to form —CN.

It is understood that substituents and substitution patterns on the compounds of the instant invention can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be readily synthesized by techniques known in the art, as well as those methods set forth below, from readily available starting materials. If a substituent is itself substituted with more than one group, it is understood that these multiple groups may be on the same carbon or on different carbons, so long as a stable structure results.

In choosing the compounds of the present invention, one of ordinary skill in the art will recognize that the various substituents, i.e. R₁, R₂, etc. are to be chosen in conformity with well-known principles of chemical structure connectivity.

In the compound structures depicted herein, hydrogen atoms, except on ribose and deoxyribose sugars, are generally not shown. However, it is understood that sufficient hydrogen atoms exist on the represented carbon atoms to satisfy the octet rule.

As used herein, and unless stated otherwise, each of the following terms shall have the definition set forth below.

-   -   A—Adenine;     -   C—Cytosine;     -   DNA—Deoxyribonucleic acid;     -   G—Guanine;     -   RNA—Ribonucleic acid;     -   T—Thymine; and     -   U—Uracil.     -   dNPP—deoxyribonucleotide polyphosphate     -   rNPP—ribonucleotide polyphosphate

“Nucleic acid” shall mean, unless otherwise specified, any nucleic acid molecule, including, without limitation, DNA, RNA and hybrids thereof. In an embodiment the nucleic acid bases that form nucleic acid molecules can be the bases A, C, G, T and U, as well as derivatives thereof. Derivatives of these bases are well known in the art, and are exemplified in PCR Systems, Reagents and Consumables (Perkin Elmer Catalogue 1996-1997, Roche Molecular Systems, Inc., Branchburg, N.J., USA).

A nucleotide polyphosphate, such as a deoxyribonucleotide polyphosphate (“dNPP”) or a ribonucleotide polyphosphate “(rNPP”), is a nucleotide comprising multiple, i.e. three, four, five, six, or more phosphates in a linear fashion bonded to its 5′ sugar carbon atom. A nucleotide polyphosphate analogue is an analogue of such a deoxyribonucleotide polyphosphate or of such a ribonucleotide polyphosphate as defined herein, differing thereform by having a tag attached thereto at a specified position. Such analogues are incorporable into a primer or nucleic acid extension strand, such as a DNA extension strand, by contacting with an appropriate nucleic acid polymerase under the appropriate nucleic acid polymerization conditions known to those in the art.

In one embodiment the dNPP is a deoxynucleotide triphosphate.

As used herein a tetranucleotide, a pentanucleotide, or a hexanucleotide, encompasses 4, 5 or 6, respectively, nucleic acid monomer residues joined by phosphodiester bonds, wherein the free terminal residue can be a nucleotide or a nucleoside. In an embodiment, the free terminal residue is a nucleoside and the other residues are nucleotides.

“Solid substrate” shall mean any suitable medium present in the solid phase to which a nucleic acid may be affixed. Non-limiting examples include chips, wells, beads, nanopore structures and columns. In a non-limiting embodiment the solid substrate can be present in a solution, including an aqueous electrolyte solution.

“Hybridize” shall mean the annealing of one single-stranded nucleic acid to another nucleic acid (such as primer) based on the well-understood principle of sequence complementarity. In an embodiment the other nucleic acid is a single-stranded nucleic acid. The propensity for hybridization between nucleic acids depends on the temperature and ionic strength of their milieu, the length of the nucleic acids and the degree of complementarity. The effect of these parameters on hybridization is well known in the art (see Sambrook J, Fritsch E F, Maniatis T. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York.). As used herein, hybridization of a primer sequence, or of a DNA extension product, to another nucleic acid shall mean annealing sufficient such that the primer, or DNA extension product, respectively, is extendable by creation of a phosphodiester bond with an available nucleotide or nucleotide analogue capable of forming a phosphodiester bond, therewith.

As used herein, unless otherwise specified, a base which is “different from” another base or a recited list of bases shall mean that the base has a different structure than the other base or bases. For example, a base that is “different from” adenine, thymine, and cytosine would include a base that is guanine or a base that is uracil.

“Primer” as used herein (a primer sequence) is a short, usually chemically synthesized oligonucleotide, of appropriate length, for example about 18-24 bases, sufficient to hybridize to a target DNA (e.g. a single stranded DNA) and permit the addition of a nucleotide residue thereto, or oligonucleotide or polynucleotide synthesis therefrom, under suitable conditions well-known in the art. In an embodiment the primer is a DNA primer, i.e. a primer consisting of, or largely consisting of, deoxyribonucleotide residues. The primers are designed to have a sequence which is the reverse complement of a region of template/target DNA to which the primer hybridizes. The addition of a nucleotide residue to the 3′ end of a primer by formation of a phosphodiester bond results in a DNA extension product. The addition of a nucleotide residue to the 3′ end of the DNA extension product by formation of a phosphodiester bond results in a further DNA extension product.

In an embodiment the single-stranded DNA, RNA, primer or probe is bound to a solid substrate via 1,3-dipolar azide-alkyne cycloaddition chemistry. In an embodiment the DNA, RNA, primer or probe is bound to a solid substrate via a polyethylene glycol molecule. In an embodiment the DNA, RNA, primer or probe is alkyne-labeled. In an embodiment the DNA, RNA, primer or probe is bound to a solid substrate via a polyethylene glycol molecule and a solid substrate is azide-functionalized In an embodiment the DNA, RNA, primer or probe is immobilized on the solid substrate via an azido linkage, an alkynyl linkage, or biotin-streptavidin interaction. Immobilization of nucleic acids is described in Immobilization of DNA on Chips II, edited by Christine Wittmann (2005), Springer Verlag, Berlin, which is hereby incorporated by reference. In an embodiment the DNA is single-stranded DNA. In an embodiment the RNA is single-stranded RNA.

In an embodiment the solid substrate is in the form of a chip, a bead, a well, a capillary tube, a slide, a wafer, a filter, a fiber, a porous media, a porous nanotube, or a column. This invention also provides the instant method, wherein the solid substrate is a metal, gold, silver, quartz, silica, a plastic, polypropylene, a glass, or diamond. This invention also provides the instant method, wherein the solid substrate is a porous non-metal substance to which is attached or impregnated a metal or combination of metals. The solid surface may be in different forms including the non-limiting examples of a chip, a bead, a tube, a matrix, a nanotube. The solid surface may be made from materials common for DNA microarrays, including the non-limiting examples of glass or nylon. The solid surface, for example beads/micro-beads, may be in turn immobilized to another solid surface such as a chip.

In an embodiment nucleic acid samples, DNA, RNA, primer or probe are separated in discrete compartments, wells or depressions on a surface or in a container.

This invention also provides the instant method, wherein about 1000 or fewer copies of the nucleic acid sample, DNA, RNA, primer or probe, are bound to the solid surface. This invention also provides the instant invention wherein 2×10⁷, 1×10⁷, 1×10⁶ or 1×10⁴ or fewer copies of the nucleic acid sample, DNA, RNA, primer or probe are bound to the solid surface.

In an embodiment the immobilized nucleic acid sample, DNA, RNA, primer or probe is immobilized at a high density. This invention also provides the instant invention wherein over or up to 1×10⁷, 1×10⁸, 1×10⁹ copies of the nucleic acid sample, DNA, RNA, primer or probe, are bound to the solid substrate.

In an embodiment the DNA polymerase is 9° N polymerase or a variant thereof, E. Coli DNA polymerase I, Bacteriophage T4 DNA polymerase, Sequenase, Taq DNA polymerase or 9° N polymerase (exo-)A485L/Y409V.

In an embodiment of the methods or of the compositions described herein, the DNA is single-stranded. In an embodiment of the methods or of the compositions described herein, the RNA is single-stranded, Phi29, or variants thereof.

In an embodiment of the methods described for RNA sequencing, the polymerase is an RNA polymerase, reverse transcriptase or appropriate polymerase for RNA polymerization as known in the art.

The linkers may be photocleavable. In an embodiment UV light is used to photochemically cleave the photochemically cleavable linkers and moieties. In an embodiment, the photocleavable linker is a 2-nitrobenzyl moiety.

The —CH₂N₃ group can be treated with TCEP (tris(2-carboxyethyl)phosphine) so as to remove it from the 3′ O atom of a dNPP analogue, or rNPP analogue, thereby creating a 3′ OH group.

Methods for production of cleavably capped and/or cleavably linked nucleotide analogues are disclosed in U.S. Pat. No. 6,664,079, which is hereby incorporated by reference.

A “nucleotide residue” is a single nucleotide in the state it exists after being incorporated into, and thereby becoming a monomer of, a polynucleotide. Thus, a nucleotide residue is a nucleotide monomer of a polynucleotide, e.g. DNA, which is bound to an adjacent nucleotide monomer of the polynucleotide through a phosphodiester bond at the 3′ position of its sugar and is bound to a second adjacent nucleotide monomer through its phosphate group, with the exceptions that (i) a 3′ terminal nucleotide residue is only bound to one adjacent nucleotide monomer of the polynucleotide by a phosphodiester bond from its phosphate group, and (ii) a 5′ terminal nucleotide residue is only bound to one adjacent nucleotide monomer of the polynucleotide by a phosphodiester bond from the 3′ position of its sugar.

Because of well-understood base-pairing rules, determining the identity (of the base) of dNPP analogue (or rNPP analogue) incorporated into a primer or DNA extension product (or RNA extension product) by measuring the unique electrical signal of the tag translocating through the nanopore, and thereby the identity of the dNPP analogue (or rNPP analogue) that was incorporated, permits identification of the complementary nucleotide residue in the single stranded polynucleotide that the primer or DNA extension product (or RNA extension product) is hybridized to. Thus, if the dNPP analogue that was incorporated comprises an adenine, a thymine, a cytosine, or a guanine, then the complementary nucleotide residue in the single stranded DNA is identified as a thymine, an adenine, a guanine or a cytosine, respectively. The purine adenine (A) pairs with the pyrimidine thymine (T). The pyrimidine cytosine (C) pairs with the purine guanine (G). Similarly, with regard to RNA, if the rNPP analogue that was incorporated comprises an adenine, an uracil, a cytosine, or a guanine, then the complementary nucleotide residue in the single stranded RNA is identified as an uracil, an adenine, a guanine or a cytosine, respectively.

Incorporation into an oligonucleotide or polynucleotide (such as a primer or DNA extension strand) of a dNPP or rNPP analogue means the formation of a phosphodiester bond between the 3′ carbon atom of the 3′ terminal nucleotide residue of the polynucleotide and the 5′ carbon atom of the dNPP analogue or rNPP analogue, respectively.

As used herein, unless otherwise specified, a base (e.g. of a nucleotide polyphosphate analogue) which is different from the type of base of a referenced molecule, e.g. another nucleotide polyphosphate analogue, means that the base has a different chemical structure from the other/reference base or bases. For example, a base that is different from adenine would include a base that is guanine, a base that is uracil, a base that is cytosine, and a base that is thymine. For example, a base that is different from adenine, thymine, and cytosine would include a base that is guanine and a base that is uracil.

As used herein, unless otherwise specified, a tag (e.g. of a nucleotide polyphosphate analogue) which is different from the type of tag of a referenced molecule, e.g. another nucleotide polyphosphate analogue, means that the tag has a different chemical structure from the chemical structure of the other/referenced tag or tags.

“Nanopore” includes, for example, a structure comprising (a) a first and a second compartment separated by a physical barrier, which barrier has at least one pore with a diameter, for example, of from about 1 to 10 nm, and (b) a means for applying an electric field across the barrier so that a charged molecule such as DNA, nucleotide, nucleotide analogue, or tag, can pass from the first compartment through the pore to the second compartment. The nanopore ideally further comprises a means for measuring the electronic signature of a molecule passing through its barrier. The nanopore barrier may be synthetic or naturally occurring in part. Barriers can include, for example, lipid bilayers having therein α-hemolysin, oligomeric protein channels such as porins, and synthetic peptides and the like. Barriers can also include inorganic plates having one or more holes of a suitable size. Herein “nanopore”, “nanopore barrier” and the “pore” in the nanopore barrier are sometimes used equivalently.

Nanopore devices are known in the art and nanopores and methods employing them are disclosed in U.S. Pat. Nos. 7,005,264 B2; 7,846,738; 6,617,113; 6,746,594; 6,673,615; 6,627,067; 6,464,842; 6,362,002; 6,267,872; 6,015,714; 5,795,782; and U.S. Publication Nos. 2004/0121525, 2003/0104428, and 2003/0104428, each of which are hereby incorporated by reference in their entirety.

In an embodiment of the molecules and the methods disclosed herein the tag is attached to the remainder of the molecule by a chemical linker which is cleavable.

In an embodiment the nanpore is in a solid-state membrane. In an embodiment the membrane is a silicon nitride membrane. In an embodiment the nanopore is a biopore. In an embodiment the pore is proteinaceous. In an embodiment the pore isan alpha-hemolysin pore. In an embodiment the pore is a graphene pore.

In an embodiment the DNA, RNA or single stranded nucleic acid is located on one side of the membrane in which the nanopore is located and the membrane is located in a conducting electrolyte solution.

Where a range of values is provided, unless the context clearly dictates otherwise, it is understood that each intervening integer of the value, and each tenth of each intervening integer of the value, unless the context clearly dictates otherwise, between the upper and lower limit of that range, and any other stated or intervening value in that stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges, and are also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding (i) either or (ii) both of those included limits are also included in the invention.

All combinations of the various elements described herein are within the scope of the invention. All sub-combinations of the various elements described herein are also within the scope of the invention.

This invention will be better understood by reference to the Experimental Details which follow, but those skilled in the art will readily appreciate that the specific experiments detailed are only illustrative of the invention as described more fully in the claims which follow thereafter.

EXPERIMENTAL DETAILS AND DISCUSSIONS

The invention disclosed herein pertains to modified nucleotides for single molecule analysis of DNA (or RNA, mutatis mutandis) using nanopores. Modifications can be made at various positions of a nucleotide, i.e. the terminal phosphate, the base, and/or the 2′, or 3′-OH to form a nucleotide analogue. After a polymerase extension reaction on a template-primer complex, the released tag-attached pyrophosphate passes through a nanopore and the resulting current blockage is monitored to determine the nucleotide base added. If the modification or tag is at the base moiety, or the 2′/3′—OH of the sugar moiety of the nucleotide, then after incorporation by DNA/RNA polymerase, the linker-tag is cleaved from the base/sugar by chemical or photochemical means and released linker-tag passes through a nanopore to identify the added nucleotide.

Nucleoside-5′-polyphosphates carrying different number of phosphate groups as linkers and modified with tags attached to the terminal phosphate of the nucleotides are designed and synthesized. After incorporation by DNA/RNA polymerase in a template-primer extension reaction, the released tag-attached polyphosphate (di-, tri-, tetra-, penta-, etc.) can be detected using a nanopore to produce sequence data. Optionally, the released tag-polyphosphates can also be treated with alkaline phosphatase to provide free tags. Using four different tags which are distinct and specific for each nucleotide base, the sequence of the template DNA or RNA can be determined.

Nucleotides carrying different number of phosphate groups or tags for the synthesis of modified nucleotides, which are efficient substrates in polymerase reactions, are provided. The released tag-attached polyphosphate is detected using a nanopore to determine conditions for design and modification of the nucleotides to achieve distinct blockade signals.

Also provided are nucleotides carrying linker-tag attached at the nucleotide base moiety, and/or the 2′/3′—OH of the sugar moiety, for DNA polymerase reaction to generate linker-tag labeled single base DNA extension product. These nucleotides are good substrates for commonly used DNA/RNA polymerases. The linker-tag attached at the extended DNA product is cleaved by chemical or photochemical means to generate the primer ready for further extension using the modified nucleotides. The released linker-tag is passed through nanopore and identified based on the difference in size, shape, and charge on the tag to produce sequence data.

As disclosed herein, these molecular tools facilitate single molecule sequencing using nanopore at single base resolution.

Here are disclosed several improvements to the nanopore approach: 1) to achieve accurate and obvious discrimination of the four bases (A, C, G and T) that make up the nucleic acid molecules; 2) to enhance and differentiate the strength of the detection signals; 3) to develop an effective method for discerning and processing the electronic blockade signals generated; 4) to control the translocation rate of nucleic acids through the pore, such as slowing down the movement of tags to improve the ability of base-to-base discrimination; and 5) to design and make new and more effective synthetic nanopores for differentiating the four different nucleotides in DNA.

The structures of four nucleotides are shown in FIG. 2 . A and G are purines, while C and T are pyrimidines. The overall molecular size of A and G is very similar, while the size of C and T is similar. Nanopores have been shown to be able to differentiate between purines and pyrimidines [Akeson et al. 1999 and Meller et al. 2000], but not be able to distinguish between individual purines, A and G, or between individual pyrimidines, C and T.

Previous studies have shown modifications of nucleoside-5′-triphosphates, including introducing more phosphate groups to produce tetra-, penta-, or hexa-phosphates, introducing dye directly to the terminal phosphate, or attaching a linker between the terminal phosphate and the dye [Kumar et al., 2006 and 2008]. Tetra- and penta-phosphates are better DNA polymerase substrates, and dye-labeled hexa-phosphate nucleotides have been developed [Kumar et al. 2005; Sood et al. 2005; Eid et al. 2009].

Nucleotide analogues which are designed to enhance discrimination of each nucleotide by modification of the nucleotides at the terminal phosphate moiety are disclosed herein. Nucleoside-5′-polyphosphates are synthesized and different tags (such as, different length/mass poly(ethylene glycol)s (PEGs), amino acids, carbohydrates, oligonucleotides, dyes or organic/inorganic molecules) are attached to the terminal phosphate group. After polymerase extension reactions, tag-attached polyphosphate moieties are generated (FIG. 3 ) and different signal specific to each base is produced when the tag-attached polyphosphate moieties pass through the nanopore. These modifications enlarge the discrimination of the bases by nanopore due to the increased size, mass or charge differences of released tagged-polyphosphate units between the four nucleotides (A, G, C and T).

The DNA translocation rate through the nanopore is reduced due to the bulkiness of the released tag-attached polyphosphates, although the translocation rate of the tags through the nanopore does not need to be reduced as long as the tags can be differentiated. Thus, the accuracy and reliability required for the base-to-base sequencing becomes achievable. Other analytical parameters in nanopore sequencing, such as concentration of the polynucleotide, magnitude of the applied voltage, temperature and pH value of the solution, are optimized in order to get the most accurate and reliable results for the detection and analysis of DNA chain.

Single-molecule approaches to sequencing allow for the possibility of deriving haplotypes for genetic studies and permitting direct sequencing of mRNAs. Among the potential single-molecule approaches for decoding the sequence of DNA or RNA molecules is the use of biological or synthetic nanopores as detectors of the individual DNA bases.

Existing sequencing-by-synthesis (SBS) approach uses cleavable fluorescent nucleotide reversible terminators (CF-NRTs) [Guo et al. 2010]. SBS method is based on the ability to pause after each nucleotide addition during the polymerase reaction and the use of specific fluorophores to discriminate among the bases. However, a major limitation of SBS for single molecule sequencing is the requirement for expensive fluorescence detectors and rapid imaging software. The method and process disclosed herein harness the advantages of SBS, especially its high accuracy, with the speed and sensitivity of the nanopore as an ionic current impedance detector.

While much research has gone into threading DNA through nanopores, with the hope of discriminating each base as it passes through due to its variable effect on the ion current, this has been very hard to achieve, both due to the speed of transmission and the effect of surrounding bases which may contribute their own effects on ions and counter ions passing through the pores [Timp et al. 2010]. The use of cyclodextrins or other ring-shaped structures in the lumen of protein pores help provide a ratcheting mechanism to slow down transit time [Astier et al. 2006], but the ability to absolutely recognize each base for sequencing as it passes remains a challenge. An alternative strategy which uses exonuclease to allow one nucleotide at a time to traverse the pore has led to single base discrimination [Clarke et al. 2009]. However, there is still difficulty in controlling the reaction time of the exonuclease for different lengths of DNA and nucleotide and the speed at which the released ions arrive at the pore with this approach.

Polymerase reaction itself displays high processivity and stable rates of base incorporation. Indeed, polymerase reactions have been used to control the movement of DNA strands through nanopores for direct base discrimination [Benner et al. 2007, Cockroft et al. 2008, Hurt et al. 2009]. During the polymerase reaction, there is release of a pyrophosphate (PPi) moiety. Therefore, if one attaches a different tag to the triphosphate for each of the four nucleotides, these could be discriminated as they are released and pass through an appropriate nanopore for DNA sequence determination. These relatively small pyrophosphate analogs, or equivalent molecules with additional positively charged groups, can reach the pore extremely rapidly. The rate of nucleotide incorporation by polymerases is approximately 1000 nucleotides per second, i.e. a millisecond per base addition, while the transport rate through the nanopore is 1 molecule per microsecond. Thus, with proper fluidics and engineering, there are no de-phasing issues to sequence DNA with our approach, nor are there difficulties with the decoding of homopolymer stretches. It has been shown that one can discriminate among a wide size range of polyethylene glycols differing by as little as one or two carbon units by the effect they have on blocking currents in nanopores [Reiner et al. 2010, Robertson et al. 2007], a resolution essentially equivalent to that obtained by a mass spectrometer. Therefore, as described below, different length PEG chains are attached to the terminal phosphate of dATP, dCTP, dGTP and dTTP. As each nucleotide is incorporated during the polymerase reaction, a specifically tagged phosphate group is released into the nanopore, yielding a distinct current blockade signal to indicate which nucleotide is incorporated. The speed of sequencing is extremely fast, limited only by the rate of the polymerase reaction. As an alternative approach for tagging the nucleotides, we also utilize different phosphate chain lengths (e.g., tri-, tetra-, and penta-phosphates).

Additionally, we also use solid-state nanopores which have advantages in terms of better control over and flexibility of fabrication, thus ensure rapid vectorial transport of tagged polyphosphates but not the nucleotide precursors or the DNA toward and through the nanopores or nanochannels. To achieve this, two important design features are incorporated. First, the precursors (tagged nucleotide polyphosphates) are synthesized with an overall neutral charge, while the cleaved tagged phosphates have an overall positive charge. By utilizing a current that attracts positive ions, the nanopores only need to discriminate the four alternative released tagged molecules. Differential charge on precursors and products are achieved by incorporate into the tags a number of lysines or arginines (positively charged) exactly balancing the number of phosphates (negatively charged). After incorporation of the α-phosphate into the growing primer, there is one more lysine than phosphate in the released product. Optionally, alkaline phosphatase can be used to cleave off all the phosphates to produce a PEG tag with a stronger positive charge. Second, to assure that the released phosphates move immediately through the nearest pore, the DNA polymerase is immobilized to the inlet of the pore, for example via a biotin-streptavidin linkage. As the DNA chain threads through the polymerase, the released tagged products only have to diffuse the same short distance to reach the nanopore.

It is also important to recognize the advantages of the bioelectronic transduction mechanism over optical approaches. For single-molecule optical transduction techniques, the signal from a single-fluorophore is typically <2500 photons/sec (corresponding to detected current levels on the order of 50 fA) at high short noise levels, requiring complex optics to try to collect every photon emitted, making scaling of the platforms to higher densities difficult. Synthesis reactions must be slowed to 1 Hz to allow sufficient integration times for these weak, noisy optical signals. The challenges to optical techniques have opened up the possibility for bioelectronic detection approaches, which have significantly higher signal levels (typically more than three orders of magnitude higher), allowing for the possibility for high-bandwidth detection with the appropriate co-design of transducer, detector, and amplifier. Signal levels for nanopores can be as high as 100 pA from alpha-hemolysin [Kasianowicz et al. 1996], 300 pA for MspA [Derrington et al. 2010], and upwards of 4 nA from solid-state nanopores [Wanunu et al. 2010].

Significant effort has been directed toward the development of nanopore technology as a bioelectronic transduction mechanism [Benner et al. 2007, Deamer et al. 2002, Kasianowicz et al. 1996, Branton 2008, Branton et al. 2008, Chen 2004, Gershow et al. 2007, Nealy 2007, Matysiak et al. 2006]. Two essential attributes of this electronic sensor give it single-molecule sensitivity. The first is the very localized (nanoscale) geometry of charge sensitivity in the pore itself. The diameter of a pore might be 2-3 nm, and due to electrolyte charge screening the measured current is highly insensitive to charge sources more than a few nanometers from the pore. Second, the nanopore sensor provides a gain through the effect the comparatively slow-moving charge a biopolymer has on a nearby concentration of higher-mobility salt ions. Nanopores, however, are extremely limited by the relatively short time biomolecules spend in the charge-sensitive region of the pore. This is directly addressed by the use of tags, which can be optimized to produce high signal levels and longer translocation events. At the same time, CMOS co-integration of these pores is exploited to dramatically improve the noise-limited bandwidths for detection in a nanopore device. Both solid-state and biological pores are supported by this platform. This solid-state integration, along with associated microfluidics, also uniquely enables the scale-up of this design to large arrays with integrated electronics for detection.

Example 1

I. Design and Synthesis of Modified Nucleotides

Effect of bulkiness of the tagged-polyphosphate on electronic blockade signals generated by a nanopore is determined using various phosphate-linked-nucleotides with different size tags or groups attached to the terminal phosphate of the nucleotide. Structures of four phosphate-tagged nucleoside-5′-polyphosphates are shown in FIG. 4 . First, a series of nucleoside-5′-tri-, tetra-, penta-, and hexa-phosphates is synthesized. In these nucleotides, the terminal phosphate is attached with a linker through which different tags, e.g. different length and mass ethylene glycols or other molecules which increases the bulkiness or charge of the released polyphosphate, are attached. These nucleotides are tested with nanopore to determine which tags or bulky groups attached to the terminal phosphate correlate to more dramatic difference in electronic blockade signal between the different bases.

1) Terminal Phosphate-Modified Nucleoside-Polyphosphates

a. Terminal Phosphate-Tagged Nucleoside-5′-Triphosphates

As shown in FIG. 5 , terminal phosphate tagged-nucleoside-5′-triphosphates can be synthesized by reacting the corresponding dNTP with DCC/DMF to give cyclic trimetaphosphate which can be opened with appropriate nucleophiles to give tag or linker attached nucleoside-5′-triphosphate. This can be used in a template-primer extension reaction and the released tag-attached pyrophosphate can be read using nanopore. Alternatively, the linker attached to the phosphate can be reacted with tag-NHS ester to provide alternate tag-attached nucleoside-5′-triphosphate.

b. Terminal Phosphate-Tagged Nucleoside-5′-Tetraphosphates

For the synthesis of terminal phosphate tagged nucleoside-5′-tetraphosphates, the corresponding triphosphate is first reacted with CDI in DMF to activate the terminal phosphate group which is then reacted with phosphoric acid or tag-monophosphate to give the tetraphosphate (FIG. 6 ). The terminal phosphate on the tetraphosphate can be further activated with CDI followed by reaction with appropriate nucleophiles to provide a linker attached tertraphosphate which can further be used to attach tags of different mass, length or bulk, such as m-dPEG-NHS ester, also shown in FIG. 6 .

c. Terminal Phosphate-Tagged Nucleoside-5′-Penta- and Hexaphosphates

Synthesis of terminal phosphate tagged nucleoside-5′-penta-and hexaphosphates follows the same principle as shown in FIG. 7 . They can be prepared either from activated triphosphates or the tetraphosphates by reacting with phosphoric acid, pyrophosphate or tag-attached phosphates. Alternatively, a linker can be attached to penta- or hexa-phosphate followed by reaction with activated NHS esters.

d. Oligo-Tag Attached Nucleoside-Polyphosphates

There are a number of issues with current approach to nanopore sequencing such as recognition of the bases as they pass through the nanopore and the speed or rate of transport to allow recognition of the nucleobase be registered. DNA passes through a α-hemolysin nanopore at a rate of 1-5 μs, which is too fast to record for single molecule sequencing experiments. Some progress has been made to overcome these issues by a variety of protein engineering strategies including the use of molecular brakes (short covalently attached oligonucleotides) [Bayley, H. 2006].

As disclosed herein, short oligonucleotides can be attached to the terminal-phosphate of a nucleoside polyphosphate by reaction of the activated terminal phosphate with the 3′-OH or the 5′-OH of the oligonucleotide. Alternatively, the 3′- or 5′-phosphate of the oligonucleotide can be activated with CDI Or Imidazole/DCC and reacted with nucleoside-5′-polyphosphates. Structures of oligo-attached nucleoside phosphates (oligo-3′ to 5′-phosphate; oligo-5′ to 5′-phosphate) are shown in FIGS. 8(a) and 8(b), respectively. The polymerase reaction by-product which is monitored by passing through the nanopore is shown in FIG. 8(c).

The rate of migration through the nanopore of the polymerase reaction by-product can be controlled by attaching oligonucleotides of different length to different nucleoside-5′-polyphosphates. For example, if nucleoside dA has 1 or 2 oligo-dA units attached, dT may have 3 oligo-dT units, dC may have 4 oligo-dC units, and dG may have 5 oligo-dG units. Different combinations of the number of oligos for each nucleotide could be used to control the transport and retention time in a nanopore.

The transport and retention time in a nanopore also can be controlled by adding different number of phosphate groups to the nucleotides. Thus the charge and mass can vary for each nucleotide polyphosphate.

Examples of Linker Tag Structure

Specific examples of reactive groups on the terminal phosphates or the nucleoside base moiety and groups with which groups can react are provided in Table 1. The reactive groups with which they can react can be present either on the linker or on the tag.

TABLE 1 Possible Reactive Substituents and Functional Groups Reactive Therewith Reactive Groups Functional Groups Succinimidyl esters Primary amino, secondary amino Anhydrides, acid halides Amino and Hydroxyl groups Carboxyl Amino, Hydroxy, Thiols Aldehyde, Isothiocyanate & Isocyanates Amino groups Vinyl sulphone & Dichlorotriazine Amino groups Haloacetamides Thiols, Imidazoles Maleimides Thiols, Hydroxy, Amino Thiols Thiols, Maleimide, Haloacetamide Phosphoramidites, Activated P. Hydroxy, Amino, Thiol groups Azido Alkyne

Tags which can be detected by nanopore are included herewith but by no means are they limited to these group of compounds. One skilled in the art may change the functional group(s) to come up with a suitable tag.

The tags include aliphatic, aromatic, aryl, heteroaryl compounds with one or more 4-8 membered rings and may optionally be substituted with halo, hydroxy, amino, nitro, alkoxy, cyano, alkyl, aryl, heteroaryl, acid, aldehyde, Azido, alkenyl, alkynyl, or other groups. These includes, poly-ethylene glycols (PEGs), carbohydrates, aminoacids, peptides, fluorescent, fluorogenic (non-fluorescent but become fluorescent after removal of protecting group) chromogenic (colorless but become colored after removal of protecting group) dyes, chemiluminiscent compounds, nucleosides, nucleoside-mono, di or polyphosphates, oligonucleotides, aryl, heteroaryl or aliphatic compounds. Some examples are given in FIG. 16 .

Structure of PEG-phosphate-labeled nucleotides and some examples of possible PEGs with different reactive groups to react with functional groups are exemplified in FIG. 17 .

Some other examples of the dyes or compounds which can be used to attach to the terminal phosphate or the base moiety of the nucleotides are provided here. By no means, these are the only compounds which can be used. These are listed here as examples and one skilled in the art can easily come up with a suitable linker-tag which can be attached to the nucleotide and detected by nanopore.

Other Examples of Suitable Tags are:

Fluorescent dyes: Xanthine dyes, Bodipy dyes, Cyanine dyes Chemiluminiscent compounds: 1,2-dioxetane compounds (Tropix Inc., Bedford, Mass.). Amino acids & Peptides: naturally occurring or modified aminoacids and polymers thereof. Carbohydrates: glucose, fructose, galactose, mannose, etc. NMPs & NDPs: nucleoside-monophosphates, nucleoside-diphosphates. Aliphatic or aromatic acids, alcohols, thiols, substituted with halogens, cyano, nitro, alkyl, alkenyl, alkynyl, azido or other such groups.

2) Base-Modified Nucleoside-5′-Triphosphates

A variety of nucleotide reversible terminators (NRTs) for DNA sequencing by synthesis (SBS) are synthesized wherein a cleavable linker attaches a fluorescent dye to the nucleotide base and the 3′-OH of the nucleotide is blocked with a small reversible terminating group [Ju et al. 2006, Guo et al. 2008 & 2010]. Using these NRTs, DNA synthesis is reversibly stopped at each position. After recording the fluorescent signal from the incorporated base, the cleavable moieties of the incorporated nucleotides are removed and the cycle is repeated.

The same type of nucleotides can also be used for nanopore DNA sequencing. As shown in FIG. 9(A), a small blocking group at 3′-OH and a tag-attached at the base linked through a cleavable linker can be synthesized. After polymerase extension reaction, both the 3′-O-blocking group and the tag from the base are cleaved and the released tag can be used to pass through the nanopore and the blockage signal monitored. Four different tags (e.g. different length and molecular weight poly-ethylenene glycols (PEGs), as shown in FIG. 9(A)) can be used, one for each of the four bases, thus differentiating the blockage signals.

Alternatively, the 3′-O-blocking group is not used because it has been shown that a bulky group or nucleotide base can prevent the DNA polymerase from adding more than one nucleotide at a time [Harris et al. 2008]. As shown in FIG. 9(B), a bulky dNMP is introduced through a cleavable linker. Thus, different dNMPs are introduced through a linker according to the original dNTP. For example, with dTTP nucleotide, a dTMP is introduced (for dATP, a dAMP; for dGTP, a dGMP and for dCTP, a dCMP is introduced). After polymerase incorporation and cleavage with TCEP, modified dNMPs are generated which are passed through the nanopore channel and detected by appropriate methods.

3) 2′- or 3′-OH Modified Nucleoside-5′-Triphosphates

Synthesis of all four 3′-modified nucleoside-5′-triphosphates can be carried out [Guo et al. 2008, Li et al. 2003, Seo et al. 2004]. 3′—O-2-nitrobenzyl and 3′-O-azidomethyl attached dNTPs (FIGS. 10A and 10B, respectively) are good substrates for DNA polymerases. After incorporation by DNA/RNA polymerase in a sequencing reaction, these 3′-O-tagged nucleotides terminate the synthesis after single base extension because of the blocking group at the 3′-OH. Further extension is possible only after cleavage of the blocking group from the 3′-O position. The 3′-O-2-nitrobenzyl group can be efficiently cleaved by UV light and 2′-O-azidomethyl by treatment with TCEP to generate the free OH group for further extension. The cleaved product from the reaction (FIG. 10C or 10D) is monitored for electronic blockage by passing through the nanopore and recording the signal. Four different substituted nitrobenzyl protected dNTPs and four different azidomethyl substituted dNTPs, one for each of the four bases of DNA, are synthesized.

II. DNA-extension Using Modified Nucleotides

1) Phosphate-Tagged Nucleotides

Terminal phosphate-tagged nucleoside polyphosphates described above are used in polymerase reactions to generate extension products. As shown in FIG. 11 , after a polymerase reaction, the released by-product of the phosphate-tagged nucleotide, tag-polyphosphate, is obtained and the extended DNA is free of any modifications. The released tag-polyphosphate is then used in an engineered nanopore by single-channel recording techniques for sequencing analysis. The released tag-polyphosphates can also be treated with alkaline phosphatase to provide free tags which can also be detected. Using four different tags for the four nucleotides (A, T, G & C) to generate four different tagged-polyphosphates which differ by mass, charge or bulk, the sequence of the DNA can be determined.

2) Base-Tagged Nucleotides with Cleavable Linkers

Base-tagged nucleotide triphosphates for DNA sequencing by synthesis (SBS) and single molecule sequencing are synthesized [Guo et al. 2008 and 2010]. The addition of large bulky groups at the 5-position of pyrimidines (C & T) and 7-position of 7-deazapurines (G & A) can block the addition of more than one nucleotide in a DNA polymerase reaction. Modified nucleotides with a cleavable linker, a bulky group, and different charges attached to the nucleotide base are synthesized. The modified nucleotides may also have a small blocking group at the 3′-OH of the nucleotides. These modified nucleotides are used in a polymerase extension reaction. As shown in FIG. 12 , after extension with the appropriate nucleotide, the linker and tag from the nucleotide base and from sugar 3′-O, if blocked, are cleaved by chemical or photochemical means and the released linker-tag is used in an engineered nanopore by single-channel recording techniques for sequencing analysis.

3) 2′- or 3′-Tagged Nucleotides with Cleavable Linkers

A linker and tag can also be attached to the 2′- or 3′-OH of nucleotides. After a polymerase extension reaction, the linker-tag is cleaved from the extended product by chemical, photochemical or enzymatic reaction to release the free 3′-OH for further extension. As shown in FIG. 13 , the released linker-tag is then used in an engineered nanopore by single-channel recording techniques for sequencing analysis.

III. DNA-Sequencing Study Using Nanopore

Discrimination of different nucleotides in DNA sequencing using nanopore is evaluated following the strategy shown in FIGS. 11-13 . To validate a nanopore's ability to distinguish the four different linker-tags in DNA, a series of experiments as shown in FIG. 14 is performed. The DNA/RNA polymerase can be bound to the nanopore and a template to be sequenced is added along with the primer. Either DNA template or primer can also be immobilized on top of the nanopore and then subsequently form a template-primer complex upon addition of a DNA polymerase. To this template-primer complex, four differently tagged nucleotides are added together or sequentially. After polymerase catalyzed incorporation of the correct nucleotide, the added nucleotide releases the tag-attached polyphosphate (in case of terminal-phosphate-labeled nucleotides) which then pass through the nanopore to generate the electric signal to be recorded and used to identify the added base. Optionally, the released tag-polyphosphate can also be treated with alkaline phosphatase to provide free tag which can also be detected by passing through the nanopore. Each tag generates a different electronic blockade signature due to the difference in size, mass or charge. In the case of base-modified or 2′/3′-modified nucleotides, after the DNA/RNA polymerase extension, the tag from the extended primer is cleaved by chemical, photochemical or enzymatic means and the electronic signature of the released tag is monitored. The shape, size, mass, charge or other properties of the tag can be adjusted according to the requirements.

As disclosed herein, signals from each of the nucleotides (FIG. 15 ) and the transitions between nucleotides of different identities are distinguished and characterized. The magnitude and duration of the blockade signatures on the event diagram are analyzed and compared with known diagrams. Thus, with these rational chemical designs and modifications of the building blocks of DNA, the use of nanopore is optimized to decipher DNA sequence at single molecule level with single base resolution.

To implement this novel strategy for DNA sequencing, an array of nanopores can be constructed on a planar surface to conduct massive parallel DNA sequencing as shown in FIG. 18 . The array of nanopores can also be constructed on a silicon chip or other such surfaces. The nanopore can be constructed from the protein with lipid bilayers or other such layers (α-hemolysin pore, Mycobacterium smegnatis porin A, MspA) [Derrington et al. 2010] or they can be synthetic solid-state nanopores fabricated in silicon nitride, silicon oxide or metal oxides [Storm et. al. 2005; Wanunu et al. 2008] or a hybrid between a solid-state pore and α-hemolysin [Hall et al. 2010].

FIG. 18 shows a schematic of array of nanopores for massive parallel DNA sequencing by synthesis. The nanopores can sense each DNA/RNA polymerase catalyzed nucleotide addition by-product (Tag-attached to the phosphate or the base and/or 2′, 3′—OH of the sugar moiety) as it passes through the nanopore. The electrical properties of different tags will distinguish the bases based on their blockade property in the nanopores. The array of nanopores shown in FIG. 18 can each read the same sequence or different sequence. Increasing the number of times each sequence is read will result in better quality of the resulting sequence data.

Example 2

I. Synthesis of PEG-Labeled-Deoxyguanosine-5′-Tetraphosphates (dG4P-PEG):

PEG-labeled-deoxyguanosine-5′-tetraphosphates (dG4P-PEGs) is synthesized according to FIG. 19 . First, 2′-deoxyguanosine triphosphate (dGTP) reacts with CDI in DMF to activate the terminal phosphate group which is then reacted with dibutylammonium phosphate to give the tetraphosphate. The terminal phosphate on this tetraphosphate is further activated with EDAC in 0.1M imidazole buffer followed by reaction with diaminoheptane to provide an amino attached tetraphosphate which is further reacted with mPEG-NHS esters to provide the required four PEG-dG4Ps. After polymerase incorporation, the net charge on the released PEG is −3 (PEG-NH-triphosphate).

II. Testing of Modified Nucleotides in Single Base Extension Reactions.

The dG4P-PEGs are characterized by MALDI-TOF mass spectroscopy as shown in Table II.

TABLE II MALDI-TOF MS Results for dG4P-PEG Calculated M.W. Measured M.W. dG4P-PEG24 1798 1798 dG4P-PEG37 2371 2374

The dG4P-PEGs are excellent substrates for DNA polymerase in primer extension. The MALDI-TOF mass spectra of the DNA extension products are shown in FIG. 20 .

Example 3—Single Molecule Detection by Nanopore of the Pegs Used To Label The Nucleotides

Poly(ethylene glycol) is a nonelectrolyte polymer that weakly binds cations (e.g., it binds K⁺ ions at K_(d)˜2 M). Thus, the net charge on the polymer depends on the mobile cation concentration and on the presence of other moieties that are chemically linked to it. It has been demonstrated that a single α-hemolysin nanopore can easily distinguish between differently-sized PEG polymers at better than monomer resolution, i.e., better than 44 g/mol [Reiner et al. 2010; Robertson et al. 2007]. That level of discrimination is made possible because the polymer reduces the pore's conductance due to volume exclusion (the pore conductance decreases with increasing polymer size) and by binding mobile cations that would otherwise flow freely through the pore [Reiner et al. 2010]. In addition, the residence time of the polymer in the pore is highly senstive to the polymer's charge, which for PEG, scales in proportion to the polymer's length. A nanopore should be able to distinguish between differently-sized PEGs that are chemically linked to other moieties. PEGs (PEG 16, 24, 37 and 49) for labeling nucleotides are tested on nanopore and generate distinct electronic blockade signatures at the single molecule level as shown in FIG. 21 .

To investigate the effect of bulkiness of the variously tagged polyphosphates on electronic blockade signals generated in the nanopore, various phosphate-linked-nucleotides are synthesized with different size polyethylene glycol (PEG) tags attached to the terminal phosphate of the nucleotide. First, as shown in FIG. 4 , we synthesize a series of nucleoside-5′-tri-, tetra-, and penta-phosphates with the terminal phosphate attached via a linker to which different tags, e.g. different length and mass PEGs or other molecules to increase the molecular size or modify the charge of the released polyphosphate, could be attached. We then test these nucleotides in polymerase reactions coupled with detection by nanopore to see which tags or bulky groups attached to the terminal phosphate produce more dramatic differences in electronic blockade signals among different bases.

I. Screen and Select 4 PEG Tags with Distinct Nanopore Blockade Signals

Recently, it has been shown that when a polyethylene glycol (PEG) molecule enters a single α-hemolysin pore, it elicits distinct mass-dependent conductance states with characteristic mean residence times [Robertson et al. 2007]. FIG. 22A shows that the conductance based mass spectrum clearly resolves the repeat units of ethylene glycol, and the residence time increases with the mass of PEG.

I.a Testing PEG for Nanopore Blockade Signatures.

Different length and molecular weight PEGs (commercially available from Quanta Biodesign Ltd or other suppliers) are selected and the nanopore blockade signals monitored, as described in Example 2. As shown in FIG. 22A PEGs of 28-48 ethylene glycol units are clearly distinguished by nanopore. Therefore, PEGs with a broad range of ethylene glycol units displaying very distinct nanopore blockade signals are selected as tags to label the nucleotides A, C, G and T. Examples are shown in FIG. 22B. Branched PEGs as tags are also evaluated as these can be modified with positive charges in a more straightforward fashion. Structures of some linear and branched PEGs are shown at the bottom of FIG. 22 .

I.b Design and Synthesis of Phosphate-Labeled PEGs Selected in I.a

In nanopore sequencing, the current blockade signals in the nanopore are generated by the PEG-phosphates released during the polymerase reaction. Thus, we design and synthesize phosphate-labeled PEGs with positively charged linkers, and test these molecules with organic (e.g., α-hemolysin) and synthetic (solid phase) nanopores to evaluate their current blockade signals. The selected PEGs are converted to their triphosphates as shown in FIG. 23 . For example, Fmoc-protected amino-butanol can be converted to the corresponding triphosphate by reacting first with phosphorous oxychloride followed by reaction with tributylammonium pyrophosphate in a one pot reaction. The triphosphate after purification is activated with DCC/DMF or CDI/DMF to provide activated triphosphate which reacts with the OH-group of the PEGs to generate PEG-triphosphates. The same scheme is applicable for both linear as well as branched PEGs. These PEG phosphates are tested in nanopores to optimize the conditions for generating distinct current blockade signals.

The polyamino acid (polylysine, polyarginine, interrupted polylysine) linkers are synthesized by standard peptide synthetic strategies; if an ester linkage to the polyphosphate chain is built in, it should be possible to use alkaline phosphatase to cleave it, resulting in more strongly positive tags for nanopore interrogation. Positive charges may also be incorporated into the PEG chains.

I.c Design and Synthesis of a Library of Terminal Phosphate-Tagged Nucleoside-5′-Triphosphates.

Terminal phosphate tagged nucleoside-5′-tri-, tetra-, and penta-phosphates are designed and synthesized. These molecules are tested in the polymerase reaction and the optimal ones are selected for nanopore detection. Terminal phosphate-tagged nucleoside-5′-tri-, tetra-, and penta-phosphates with a variety of tags, including small or large polylysines, amino acids, a variety of negatively or positively charged dyes, such as Energy Transfer dyes, and ethylene glycol units, have been shown to be accepted by DNA polymerases as excellent substrates for primer extension [Kumar et al. 2006 and 2008; Sood et al. 2005; and Eid et al. 2009].

I.c.1 Design and Synthesis of Terminal Phosphate-Tagged Nucleoside-5′-Triphosphates.

As shown in FIG. 24 , terminal phosphate tagged-nucleoside-5′-triphosphates is synthesized by reacting the corresponding dNTP with DCC/DMF to yield a cyclic trimetaphosphate which can be opened with nucleophiles to generate a tag or linker attached nucleoside-5′-triphosphate. In addition, the linker attached to the phosphate can be reacted with PEG-NHS esters to provide alternate PEG-attached nucleoside-5′-triphosphates. The resulting terminal phosphate-tagged nucleoside-5′-triphosphate is used in the template-primer extension reaction and the released tag-attached pyrophosphate is detected and differentiated by its specific nanopore current blockade parameters.

I.c.2 Design and Synthesis of Terminal Phosphate-Tagged Nucleoside-5′-Tetraphosphates.

For synthesis of terminal phosphate tagged nucleoside-5′-tetraphosphates, the corresponding triphosphate is first reacted with CDI in DMF to activate the terminal phosphate group which is then reacted with phosphoric acid or tag-monophosphate to give the tetraphosphate as shown in FIG. 25 . The terminal phosphate on the tetraphosphate can be further activated with EDAC in 0.1M imidazole buffer followed by reaction with an appropriate nucleophile to provide a linker attached tetraphosphate which can be used to attach tags of different mass, length or charge, such as m-PEG-NHS esters. In this case, four trimethyllysines are used to neutralize the charge of four phosphates. After polymerase incorporation, the net charge on the released PEG is +1 or, if treated with alkaline phosphatase, +4, which can be detected by the nanopore.

I.c.3 Design and Synthesis of Terminal Phosphate-Tagged Nucleoside-5′-Penta-Phosphates.

Synthesis of terminal phosphate tagged nucleoside-5′-penta-phosphates follows the same principle as shown in FIG. 26 . They can be prepared either from activated triphosphates or tetraphosphates by reacting with phosphoric acid, pyrophosphate or tag-attached phosphates. Alternatively, a linker can be attached to the pentaphosphate followed by reaction with activated NHS esters.

The terminal phosphate tagged nucleoside polyphosphates described above are used in the polymerase reaction to generate extension products. Following the scheme shown in FIG. 11 the performance of the terminal phosphate tagged nucleoside polyphosphates in polymerase extension are evaluated. We first perform a single base extension reaction and characterize the DNA extension product by MALDI-TOF mass spectroscopy to evaluate incorporation efficiency. After establishing optimized reaction conditions, we immobilize the template on magnetic beads and repeat the single base extension reaction, after which the released polyphosphate-tags are isolated from the solution for detection using a single nanopore. This reaction is performed continuously to evaluate all 4 nucleotides (A, C, G, and T) and their corresponding released tags detected by the nanopore. Continuous polymerase reaction with the polyphosphate-tag nucleotides and the clear distinction of the released polyphosphate-tag by nanopore establish the feasibility of the approach.

As shown in FIG. 11 , after the polymerase reaction, the released by-product of the phosphate-tagged nucleotide (tag-polyphosphate) is obtained and the extended DNA strand is free of any modifications. This is advantageous because any scars remaining on growing DNA chains can affect their ability to be recognized by polymerase with increasing nucleotide additions, eventually terminating further DNA synthesis. The released tag-attached polyphosphate are assayed in the nanopore to evaluate sequencing sensitivity and accuracy. In initial experiments we test the tags for their blockade signals before running SBS reactions. DNA sequence can be determined if different tags for the four nucleotides are used to generate four different tagged-polyphosphates which differ by mass, charge or bulk, and yield 4 distinct blockade signals.

II. Detection of the Released Tagged Phosphates by Protein Nanopores

We use a single α-hemolysin nanopore to detect PEGs that are linked to nucleotides attached via a multi-phosphate linker and the same polymer after the nucleotide/ribose moiety has been cleaved by the DNA polymerase reaction. Each of the four different DNA bases is linked to a PEG polymer with a unique length. Thus, each base that is removed from the PEG by the polymerase is identified. Because the unreacted nucleotides cannot be separated from the released tagged polyphosphates, especially in real time situations, we take advantage of the method's extreme sensitivity to molecular charge to discriminate between the released reaction product and the starting material. We measure single α-hemolysin conductance using conical glass supports [White et al., 2006 and 2007] which allow data collection at 100 kHZ and ˜4 pA RMS noise. We measure the blockade depth and residence time distributions of both the tagged nucleotides and tagged products over a wide range of transmembrane potentials to determine optimum conditions for nucleotide discrimination and to extend our current theoretical understanding of PEG-nanopore interactions [Robertson et al. 2007] to molecules with fixed charges. Characterization and theoretical understanding permit the unambiguous identification of the nucleotides incorporated into polynucleotides by polymerase. Thus, with these rational chemical designs and modifications of the building blocks of DNA, we optimize the use of nanopores to decipher DNA at the single molecule level with single base resolution in protein or synthetic nanopores.

Example 4—Fabrication of a Single Solid-State Nanopore for Single Molecule Sequencing

The transition from a protein nanopore to a solid state nanopore makes the fabrication of high-density nanopore arrays possible, a key step for yielding a high-throughput single molecule electronic DNA sequencer. Here, an integrated single solid state nanopore platform is developed to characterize the tagged nucleotides in the polymerase reaction based on the knowledge gained from the protein nanopore.

Integrated Nanopore Platform.

We developed specialized integrated low-noise CMOS electronics, which when integrated with solid-state nanopores, deliver significant performance advantages over “standard” measurement techniques which employ external electrophysiological amplifiers, such as the Axopatch 200B. These advantages come from exploiting capacitive (rather than resistive) feedback in a custom integrating amplifier design. DC current, which is characteristic of this and other bioelectronic interfaces, is removed with a low-noise current source operating in a DC servo loop. Reduced amplifier input capacitances and reduced parasitic capacitances associated with co-integration improve noise performance at high frequencies, enabling bandwidths approaching 1 MHz for solid-state pores. Such high temporal resolution, when combined with the tags developed, will provide high flexibility for tuning this platform for high sensitivity and real-time performance.

Use of this CMOS-integrated nanopore (CNP) integrated circuit in either a two-chip or one-chip configuration as shown in FIG. 27 . In the former case, the pore is packaged together with the CNP as shown in FIG. 27B. In the latter, the pore is fabricated directly into the CNP as shown in FIG. 27C with fluidics on either side of the chip. In both cases, the cis electrode, which connects to the input of the amplifier, is integrated directly on the surface of the CNP. The one-chip configuration has the advantage of being easily scalable to a multiplexed platform at the cost of additional fabrication complexity. The ability to post-process fabricated CMOS dice (which are no more than 5 mm on a side) is a unique capability established over the last five years [Huang et al. 2011, Lei et al. 2008, and Levine et al. 2009]. This approach completely leverages existing foundry process flows rather than requiring new process development.

The one-chip fabrication approach proceeds by adapting standard solid-state nanopore fabrication techniques [Rosenstein et al. 2011]. In areas of the die reserved for the sensors, all metals have been blocked, leaving a thick stack of alternating glass fill and silicon nitride capping layers. The majority of the dielectric stack is etched using an inductively-coupled CHF₃ plasma. After depositing and patterning a PECVD Si₃N₄ etch mask on the back of the die, localized openings in the silicon substrate are made using an anisotropic potassium hydroxide etch. A short dip in buffered hydrofluoric acid is then used to isolate a single 50 nm layer of silicon nitride from the original dielectric stack as a suspended membrane. Finally, nanopores are drilled through these nitride membranes with a high resolution transmission electron microscope.

The measured noise of this system is shown in FIG. 28A, alongside a measurement of the baseline noise for a comparable configuration of the Axopatch 200B. For the highest bandwidth supported by the Axopatch (B=100 kHz), the integrated amplifier has a noise floor of 3.2 pA_(RMS), compared to 9 pA_(RMS) for the Axopatch. At the highest bandwidth characterized for the integrated amplifier (B=1 MHz), the noise level is 27 pA_(RMS), in contrast with 247 pA_(RMS) modeled by extrapolating the Axopatch response beyond its supported range (approximately a factor-of-ten lower noise). As a point of comparison, for a 1 nA signal, only about 6250 ions are transported through the pore in 1 us. An input-referred noise level of 27 pAV_(RMS) for integrated amplifier allows resolution of as few as 150 ions in this interval.

It is also important to note this superior electrical performance is obtained with an integrated amplifier that consumes an area of only 0.2 mm² on a CMOS chip compared with a rack-mounted Axopatch amplifier, demonstrating the significance of the innovative electronics. When a nanopore is connected to the amplifier input, the introduction of 1/f noise and membrane capacitance raises the noise spectrum above the open-headstage baseline. FIG. 28 b shows a typical noise spectrum in this case, demonstrating noise floors of only 10 pA_(RMS) and 163 pA_(RMS) for bandwidths of 100 kHz and 1 MHz, respectively. Measured comparisons are shown with the Axopatch up to 100 kHz for the same nanopore. At 100 kHz, there is more than a factor-of-two reduction in input-referred noise power for the CNP. If the Axopatch could be measured at higher bandwidths, there would be a factor-of-six noise power difference at 1 MHz.

This platform also allows the integration of biological nanopores, providing even more flexibility. Biological nanopores are created in lipid membranes (typically 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)) formed over a hole in a teflon membrane between two fluid cells. The surface must be sufficiently hydrophilic for the membrane to form from unilamellar vesicles. The conductance between the two chambers of the cell is monitored while the membrane protein is added to one of the cells, which is immediately flushed once incorporation is detected. The membranes used to fabricate the nanopores can also be used as solid supports for lipid bilayers with the drilling of larger holes into the membranes, over which the lipid bilayer is formed [Clarke et al. 2009; Benner et al., 2007; Hou et al., 2009; and Wang et al. 2011]. Planar bilayer lipid membranes (BLMs) have been engineered with different protein channels on patterned solid supports with nanopatterned holes (˜100 nm in diameter), as well as tethering them directly on gold through a self-assembled monolayer assembly [Axelrod et al., 1976, Bultmann et al. 1991, Dutta et al. 2010, Jenkins et al. 2001, Nam et al. 2006, Palegrosdemange et al. 1991, Shen et al. 2009, Srinivasan et al. 2001, Yang et al. 2003, Yin et al. 2005]. Moreover, it has been shown that formation of contiguous BLMs with a diffusion coefficient of 4 μm²/s on nanopatterned substrates; BLMs formed on SAM-gold assemblies yielded a coefficient of 0.8 μm²/s. Both fall within the ideal diffusion range of 0.1-10 μm²/s representative of well-formed BLMs [Axelrod et al. 1976, Bultmann etal. 1991]. Electrical characterizations of these BLMs indicate a high impedance membrane with a 1.4 GW-mm² resistance, making it amenable for further electrical analysis of biological nanopores formed in the membrane [Oliver et al. 1994, Shi et al. 2000, Wiehelman 1988].

Immobilization of Polymerase to Nanopore-Bearing Surfaces

The size of the polymerase is about 5 nm×5 nm. One polymerase is positioned near the entrance to each nanopore. To accomplish this for the solid-state nanopores, it is necessary that (1) a unique position on the surface be modified with functional groups during CMOS fabrication to bind the polymerase; (2) that the sites be small enough that only one polymerase molecule can bind; (3) that they be far enough apart that there is little possibility of diffusion of the released tagged polyphosphates to a nearby channel; and (4) that the cross-linking agent be sufficiently flexible that the enzyme is functionally intact. Polymerase tethering is accomplished by combining a patterned attachment point with the use of an appropriate concentration of polymerase solution during incubation such that at most one enzyme molecule is attached.

Establishment of the appropriate tether point for the polymerase is accomplished by exploiting existing fabrication approaches for solid-state nanopores. Typically, to maximize the transduction signals, these pores are created by thinning a supported Si₃N₄ membrane using e-beam lithography to define a window which is subsequently thinned with a plasma etch (e. g. SF₆). The nanopore is then drilled in the thinned region using e-beam ablation. The well created by this window (FIG. 29 ) creates a natural place to tether the polymerase, guaranteeing close proximity to the nanopore entrance. Prior to etching the thinned window, the original membrane can be augmented with a buried epitaxial layer of attachment material. Once the window is etched, this can become a selective sidewall region for polymerase attachment. Attachment materials include silicon dioxide or gold. There may be limited selectivity with silicon dioxide, however, because an oxide can also form on the silicon nitride surface under appropriate conditions.

In principle, with silicon dioxide surfaces, biotin-streptavidin linkages can be used [Korlach et al. 2008 and 2010], utilizing biotinylated PEG molecules on the silica patches and incubate biotin-end labeled polymerase in the presence of streptavidin. The remainder of the surface is passivated with polyvinylphosphonic acid. Due to the concerns raised above, it is preferable instead to modify the gold surface with an alkanethiol self-assembled monolayer (SAM) functionalized with amino groups [Love et al. 2005]. These can be easily modified to NHS esters for attachment to amino groups on the polymerase. The thickness and homogeneity of the layer is determined by ellipsometry or atomic force microscopy.

Development of 5′-Modified Nucleotides with Positively Charged Linkers

A system for rapid diffusion of the released tags toward the pores while the precursor nucleotides and DNA are repelled by the pores is generated. The tagged nucleotides are engineered so that after incorporation into the DNA, the tag released from the nucleoside has a cumulative positive charge while the intact tag-nucleotides remain neutral. This allows actively gating the released tag specifically through the detection channel, if the channel is negatively charged according to known methods [Wanunu et al. 2007]. As all other free molecules present in the reaction mix (primers, unreacted nucleotides, template), other than the tag, are negatively charged, only the released tag carrying positive charge is attracted into the channel, increasing the specificity of detection and reducing noise. A different number of charged groups can be used on different tags, depending on the specific nucleotide base. Thus the cumulative charge of the tag along with its size can be used for base discrimination. After incorporation and release of the tag, if the polyphosphate is deemed to mask the positive charge, it can be removed using secondary reactions (for example, alkaline phosphatase immobilized at a second downstream site in the pore). The positively charged tag can be gated into the negatively charged channel for detection and recognition.

Diffusion and Drift

A critical aspect of this sequencing system is the reliable and timely capture of each nucleotide's released tag by the adjacent nanopore. Conditions must be engineered such that tags are captured quickly and in the correct order. Additionally, the capture rate of unincorporated tags should be minimized, and interference from adjacent channels should be negligible. Creating the well at the entrance of the pore (as shown in FIG. 29 ) assists this process, which also depends on close proximity of the polymerase to the nanopore opening. Analysis of nanopore capture processes generally considers a radially symmetric process surrounding the pore. Geometry dictates that in the absence of an electric field, a molecule tends to diffuse farther from a pore, opposing the electrostatic attraction. With a voltage gradient, there exists a critical distance L at which molecular motion due to diffusion and electrophoresis are equal [Gershow et al. 2007]. This critical distance is a function of the ionic current (I) and electrolyte conductivity (θ), as well as the diffusion constant (D) and mobility (μ) of the analyte molecule,

$L = {\frac{\text{?}}{\text{?}}{\frac{\mu}{D}.}}$ ?indicates text missing or illegible when filed

Capture is a statistical process, but approximately 50% of molecules at a distance L is captured. This likelihood increases for shorter distances, and exceeds 90% for d<L/3. During this process, molecules typically are captured in a timescale on the order of

$t_{capture} = {\frac{L^{2}}{2D}.}$

By placing the polymerase within L/3 of the nanopore, nearly all molecules are captured. It also ensures that t_(capture) is significantly faster than the polymerase incorporation rate, to capture bases in the correct order.

An approximate value for the diffusion coefficient of 25-unit PEG molecules in water is D=3e−10 m²/s [Shimada et al. 2005], which is on the same order of magnitude as a similar-length ssDNA fragment [Nkodo et al. 2001]. Assuming validity of the Nernst-Einstein relation (although this does not always hold true for polymers), the mobility can be estimated as a function of the diffusion constant and net charge (Q),

$\mu \approx {\frac{QD}{\text{?}}.}$ ?indicates text missing or illegible when filed

For these estimates, then, with I=5 nA in 1M KCl—see the following Table.

+1e +4e 50% capture 2.1 nm  5.8 nm 90% capture 0.7 nm  1.9 nm t_(capture) 7.1 ns  114 ns

Example 5—Fabricate an Array of Solid-State Nanopores

In addition to improved performance, only with the integrated electronics is it possible to produce massively parallel nanopore arrays. This involves the one-chip topology shown in FIG. 27C in which nanopores are integrated directly into the CMOS die with fluidics on either side of the chip. The approach for integrating multiple pores is also shown in FIG. 27C. In this case, wells of SU-8 photoresist are used to isolate individual nanopores from each other. This is an approach similar to that of Rothberg et al. 2011. In Rothberg et al., however, the wells can still remain “connected” by the solution reservoir above the chip. In present case, since electrical isolation is necessary between the cis reservoirs, a PDMS cap is used to seal the wells for measurement after the introduction of reagents as shown in FIG. 27C. 64 solid-state nanopores are integrated onto the same 5-mm-by-5-mm die. The current integrating amplifier design, which would have to be duplicated at each pore site, is only 250 um by 150 um, but additional space has to be left for the fabrication of the pore itself. As fabrication techniques are further developed to reduce the chip area, this can be easily scaled to an array of 16-by-16 electrodes.

Example 6—Pyrosequencing Using Phosphate-Tagged Nucleotide And Nanopore Detection

Pyrosequencing is sequencing by synthesis (SBS) method which relies on the detection of pyrophosphate that is released when a nucleotide is incorporated into the growing DNA strand in the polymerase reaction [Ronaghi et al. 1998]. In this approach, each of the four dNTPs is added sequentially with a cocktail of enzymes, substrates, and the usual polymerase reaction components. If the added nucleotide is complementary to the first available base on the template, the nucleotide will be incorporated and a pyrophosphate will be released. Through an enzyme cascade, the released pyrophosphate is converted to ATP, and then turned into a visible light signal by firefly luciferase. On the other hand, if the added nucleotide is not incorporated, no light will be produced and the nucleotide will simply be degraded by the enzyme apyrase. Pyrosequencing has been applied successfully to single nucleotide polymorphism (SNP) detection and DNA sequencing. A commercial sequencing platform was developed combining pyrosequencing and DNA template amplification on individual microbeads for high-throughput DNA sequencing [Margulies et al. 2005]. However, there are inherent difficulties in pyrosequencing for determining the number of incorporated nucleotides in homopolymeric regions (e.g. a string of several T's in a row) of the template. Beside this, there are other aspects of pyrosequencing that still need improvement. For example, each of the four nucleotides has to be added and detected separately. The accumulation of undegraded nucleotides and other components could also lower the accuracy of the method when sequencing a long DNA template.

This is a modified pyrosequencing approach which relies on the detection of released tag- or tag-phosphates during polymerase reaction. In this approach, phosphate-tagged nucleotides are used in polymerase catalyzed reaction on a template-primer complex. Upon incorporation of the tagged-nucleotides, the phosphate-tag moiety is released, which can be detected by passing through a nanopore. The same tag can be used on each nucleotide or a different molecular weight and length tag (such as PEGs) can be used. It has been shown that polyethylene glycols (PEGs) of different length and mass can be resolved at single-molecule sensitivity when passed through hemolysin nanopore [Robertson et al. 2009].

An α-hemolysin channel could be used to detect nucleic acids at the single molecule level [Kasianowicz et al. 1996]. The monomeric polypeptide self-assembles in a lipid bilayer to form a heptameric pore, with a 1.5 nm-diameter limiting aperture. In an aqueous ionic salt solution, the pore formed by the α-hemolysin channel conducts a strong and steady ionic current when an appropriate voltage is applied across the membrane. The limiting aperture of the nanopore allows linear single-stranded but not double-stranded nucleic acid molecules (diameter ˜2.0 nm) to pass through. The polyanionic nucleic acids are driven through the pore by the applied electric field, which blocks or reduces the ionic current. This passage generates a unique electronic signature. Thus a specific event diagram, which is the plot of translocation time versus blockade current, will be obtained and used to distinguish the length and the composition of polynucleotides by single-channel recording techniques based on characteristic parameters such as translocation current, translocation duration, and their corresponding dispersion in the diagram. Four PEG tags, which have been shown to yield distinct current blockade signals in nanopores, are selected to couple with four nucleotides (A, C, G, T) at the terminal phosphate. These novel nucleotide analogs are used in a polymerase reaction and use nanopores to detect the released tags for decoding the incorporated bases as shown in FIG. 14 .

There are several advantages to this approach:

-   -   1) Avoid the use of many different enzymes (saves cost and         complexity).     -   2) Addition of single tag-attached nucleoside polyphosphate         sequentially or all four nucleotides with different tags         attached to each nucleotide.     -   3) Use of PEGs as tags which can be detected by nanopore at a         single unit resolution.     -   4) Real time Single molecule detection sequencing as the tag         passes through the nanopore.     -   5) Massively parallel sequencing, low cost and high throughput.

As shown in FIG. 14 , DNA polymerase is immobilized to the nanopore and the template-primer along with the PEG-tagged nucleotides is added. On incorporation of the correct PEG-tagged nucleotide, the released PEG-phosphates pass through the nanopore and the electronic blockade signal is measured. Different length PEGs have different blockade signals, thus, 4 different PEGs can be used for 4 different nucleotides.

The nucleotides can be added one at a time, if the correct nucleotide is added it gives a distinct blockade signal. However, if the nucleotide is not complementary to the template nucleic acid base, it will not be incorporated and thus no signal detected. In a massive parallel way high density array of micro/nano wells to perform the biochemical process can be constructed. Each micro/nano-well holds a different DNA template and nanopore device. The released PEGs are detected at single-molecule sensitivity.

General methods for synthesis of TAG-labeled-nucleoside-5′-polyphosphate is shown in FIG. 30 . Terminal-phosphate-labeled-nucleoside-5′-tri, tetra-, penta-, or hexa-phosphates can be synthesized starting from the corresponding nucleoside-5′-triphosphates (NTP). Thus, triphosphate is first activated with DCC/DMF which can be directly reacted with the TAG-nucleophile to give TAG-attached-NTP or it can be reacted with a linker nucleophile to which a TAG-NHS or appropriately activated TAG can be reacted to provide TAG-linker-attached NTP. For the synthesis of TAG-attached nucleoside tetraphosphates (N4P) or pentaphosphates (N5P), the activated triphosphate is first reacted with phosphoric acid or pyrophosphate to give tetra- and penta-phosphate, respectively, which can be reacted with linker nucleophile followed by the reaction with appropriate activated TAGs.

Synthesis of PEG-labeled nucleotides are discussed above in Examples 2 and 3. The PEG-labeled nucleotides have −3, −4, −5, or −6 charges based on the use of tri, tetra-, penta-, or hexa-phosphates. After polymerase catalyzed primer-extension reaction, the net charge on the released PEG-tags will be one less (−1) than the starting PEG-nucleotide which is enough to distinguish by the nanopore ionic blockade signal (unreacted PEG-nucleotide is also bulkier than the released PEG-phosphates, thus different ionic blockade signal). Alternatively, if alkaline phosphatase is present in the reaction mixture, the released PEG will be neutral (the free phosphate groups are hydrolyzed by alkaline phosphatase). The released PEG-tags can also be made positively charged as shown below so that they can be easily detected by nanopores. Similarly, they can also be made highly negatively charge.

Synthesis of Positively Charged TAG-Attached-Nucleoside-Polyphosphates:

The positively charged TAG-attached nucleoside-polyphosphates are synthesized as shown in FIG. 25 . First, a positively charged trimethyl-(lysine)_(n)-glycine amino acid (K[(Me)₃]_(n)-Gly) is reacted with the PEG-NHS ester and then activated to form the PEG-K[(Me)₃]_(n)-Gly-NHS ester. This activated ester is reacted with the amino-terminated nucleoside-polyphosphate as shown in FIGS. 19 and 25 . The net charge on the nucleoside-tetraphosphate is neutral but after polymerase incorporation, the released PEG has a +1 positive charge and if alkaline phosphatase is added to the reaction cocktail, the net charge on the released PEG is +4. Thus the released TAG can be easily separated and identified by passing through the nanopore.

Synthesis of 3′-Blocked-PEG-Attached-Nucleoside-Polyphosphates for Sequencing by Synthesis with Nanopore Detection.

The synthesis of 3′-blocked-nucleoside-polyphosphates essentially follows the same route as shown for TAG-attached nucleoside-polyphosphates, except that the starting nucleoside-5′-triphosphate is 3′-O-blocked-dNTP. As shown in FIG. 31 , 3′-O-azidomethyl-dNTP (6) is first reacted with CDI or DCC/DMF followed by reaction with phosphoric acid (tetraphosphate) or pyrophosphate (pentaphosphates). This is reacted after purification with the appropriate nucleophile to provide amino-terminated phosphate which is then reacted with the appropriate PEG-NHS ester (neutral, positively charged or negatively charged) to provide required 3′-O-blockade-PEG-attached-nucleoside-polyphosphate.

Sequencing scheme with PEG-nucleotides and nanopore detection (many copies of a DNA molecule are immobilized on a bead and sequential addition of one PEG-nucleotide at a time).

As shown in FIG. 32 , the DNA molecules are immobilized on a bead. Thus each bead has many copies of the same DNA molecule. The bead is added to a micro/nano-well which is attached to a nanopore. The DNA forms the complex with the DNA polymerase which is either attached to the nanopore or added to the micro/nano well along with the PEG-attached nucleotide. The nucleotides can be added one at a time, if the correct nucleotide is added it is incorporated and release a PEG-Tag which gives a distinct blockade signal when passed through a nanopore. However, if the nucleotide is not complementary to the template nucleic acid base, it will not be incorporated and thus no signal detected. In this case, the same length and molecular weight PEG can be used on all four nucleotides, or, if desired, four different PEGs can also be used. Thus, addition of nucleic acid base can be easily detected by the nanopore blockade signal at single-molecule sensitivity.

Sequencing by synthesis with 3′-O-blocked-PEG-nucleotides and nanopore detection (many copies of a DNA molecule are immobilized on a bead and simultaneous addition of all four 3′-O-blocked-PEG-nucleotides).

The homopolymeric regions of the DNA can be corrected sequenced using this approach. Thus, if the 3′-OH group of the nucleotide is blocked by a reversible moiety, the DNA synthesis will stop after addition of only one nucleotide. The synthesis can be continued after the removal of the blocking group to generate a free 3′-OH group. As shown in FIG. 33 all four different size PEG-attached-3′-O-azidomethyl-nucleotides can be added to the reaction micro/nano-well and whenever a correct nucleotide is incorporated, the released PEG-tag is read by passing through the nanopore and ionic signal detected. Because 3′-OH group is blocked only one nucleotide is added at one time. This 3′-O-blocked group can be cleaved by TECP treatment and thus free OH group is ready for further nucleotide incorporation. By repeated nucleotide addition and cleavage, homopolymeric region can be correctly and easily sequenced.

Massively Parallel Pyrosequencing Using Nanopores:

As shown in FIG. 34 , in a massive parallel way high density array of micro wells to perform the biochemical process can be constructed. Each micro/nano-well holds a different DNA template and nanopore device. The released PEGs are detected at single-molecule sensitivity.

Summary of Experiment:

-   -   1) Any TAG of different size, length, molecular weight, charge         attached to the terminal phosphate of the nucleotide which can         be detected by nanopore after polymerase incorporation.     -   2) TAG attached to the tri-, tetra-, penta-, hexa-phosphates.     -   3) Electronic Detection     -   4) Group of DNA molecules attached to the bead or solid surface         and single-molecule detection sensitivity (High density and high         sensitivity).     -   5) Easily sequenced homopolymeric region by using         TAG-attached-3′-O-blocked nucleotides.     -   6) Add one TAG-nucleotide per cycle.     -   7) Add all four reversibly tagged-nucleotides together for         sequencing homopolymeric regions.     -   8) High sensitivity, accuracy and speed.     -   9) Massive parallel sequencing.

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1-43. (canceled)
 44. A compound having the structure:

wherein the tag is polyethylene glycol, an amino acid, a carbohydrate, a dye, mononucleotide, dinucleotide, trinucleotide, tetranucleotide, pentanucleotide or hexanucleotide, wherein R₁ is OH, wherein R₂ is H or OH, wherein X is O, NH, S or CH₂, wherein Z is O, S, or BH₃, wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine, and wherein n is 1, 2, 3, or
 4. 45. The compound of claim 44, wherein R₂ is H.
 46. The compound of claim 44, wherein R₂ is OH.
 47. A compound having the structure:

wherein in each structure n is, independently, 1, 2, 3 or 4, and m is, independently, an integer from 0 to 100, and wherein when m is 0 the terminal phosphate of the dNTP is bonded directly to the 3′ O atom of the nucleoside shown on the left hand side of the structure, wherein R₁ is —OH, or —O—CH₂N₃, and R₂ is H or OH.
 48. The compound of claim 47, wherein m is from 0 to
 50. 49. The compound of claim 47, wherein m is from 0 to
 10. 50. The compound of claim 47, wherein R₁ is —OH.
 51. The compound of claim 47 or claim 50, wherein R₂ is —H.
 52. The compound of claim 47 or claim 50, wherein R₂ is —OH.
 53. A compound having the structure:

wherein m an integer from 0 to 100, and wherein the compound comprises a single type of base, and wherein the base is adenine, guanine, cytosine, uracil or thymine or a derivative thereof of each.
 54. The compound of claim 53, wherein m is from 0 to
 50. 55. The compound of claim 53, wherein m is from 0 to
 10. 56. The compound of claim 53 having the structure:

wherein m is an integer from 0 to
 100. 57-86. (canceled)
 87. The compound of claim 44, wherein the tag is polyethylene glycol.
 88. The compound of claim 87, wherein the tag has the following structure:

wherein W is an integer between 0 and
 100. 89. The compound of claim 44, wherein the tag has the following structure:

wherein R is NH₂, OH, COOH, CHO, SH, or N₃, and W is an integer from 0 to
 100. 90. The compound of claim 44, wherein the net charge on the tagged nucleoside polyphosphate is neutral.
 91. The compound of claim 44, wherein the tag has a positive charge.
 92. A composition comprising at least four deoxynucleotide polyphosphate (dNPP) analogues, each having a structure selected from the structures set forth in claim 44, wherein each of the four dNPP analogues comprises a type of base different from the type of base of the other three dNPP analogues.
 93. The composition of claim 90, wherein each of the four dNPP analogues has a polyethylene glycol tag which is different in size from the polyethylene glycol tags of each of the other three dNPP analogues. 